Lipid-Gold-Nanoparticle Hybrid-Based Gene Delivery

被引：55

作者：

Rhim, Won-Kyu ^{[1
]}

Kim, Jin-Soo ^{[1
]}

Nam, Jwa-Min ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea

来源：

SMALL | 2008年 / 4卷 / 10期

关键词：

gene delivery; gold; lipids; nanoparticles; non-viral vectors;

D O I：

10.1002/smll.200800628

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Lipid-DNA-AuNP (L-DNA-AuNP) hybrid-based gene delivery system was devised for better transfection efficiency and reduced cytotoxicity by taking advantages of AuNPs and liposomes as gene-delivery vehicles. pDNA-AuNPs were modified with cationic liposomes to form L-pDNA-AuNP hybrids and its structures were characterized using a cryo-transmission electron microscope (cryo-TEM). The transfection results show that the lipid-AuNP hybrid system has much better transfection efficiency than the liposome-based system. Dehydrogenases in viable cells induce a change in color, while liposome-based transfection system shows the highest cytotoxicity. It is also seen that only the L-pDNA-AuNP hybrid-based gene delivery system has both high delivery efficiency and low cytotoxicity. Less lipid is needed in the L-pDNA-AuNP system for efficient pDNA deliver than the lipid-pDNA system.

引用

页码：1651 / 1655

页数：5

共 35 条

[1] A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles [J].

Akamatsu, K ;

Kimura, M ;

Shibata, Y ;

Nakano, S ;

Miyoshi, D ;

Nawafune, H ;

Sugimoto, N .

NANO LETTERS, 2006, 6 (03) :491-495

[2] FUNCTIONAL-GROUP CONTRIBUTIONS TO DRUG RECEPTOR INTERACTIONS [J].

ANDREWS, PR ;

CRAIK, DJ ;

MARTIN, JL .

JOURNAL OF MEDICINAL CHEMISTRY, 1984, 27 (12) :1648-1657

[3] Polymeric nano- and microparticle technologies for oral gene delivery [J].

Bhavsar, Mayank D. ;

Amiji, Mansoor M. .

EXPERT OPINION ON DRUG DELIVERY, 2007, 4 (03) :197-213

[4] Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing [J].

Cai, D ;

Mataraza, JM ;

Qin, ZH ;

Huang, ZP ;

Huang, JY ;

Chiles, TC ;

Carnahan, D ;

Kempa, K ;

Ren, ZF .

NATURE METHODS, 2005, 2 (06) :449-454

[5] Harmful potential of viral vectors fuels doubts over gene therapy [J].

Check, E .

NATURE, 2003, 423 (6940) :573-574

[6] Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity [J].

Connor, EE ;

Mwamuka, J ;

Gole, A ;

Murphy, CJ ;

Wyatt, MD .

SMALL, 2005, 1 (03) :325-327

[7] The dawning era of polymer therapeutics [J].

Duncan, R .

NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (05) :347-360

[8] Nanopartide-aptamer bioconjugates: A new approach for targeting prostate cancer cells [J].

Farokhzad, OC ;

Jon, SY ;

Khademhosseini, A ;

Tran, TNT ;

LaVan, DA ;

Langer, R .

CANCER RESEARCH, 2004, 64 (21) :7668-7672

[9] LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].

FELGNER, PL ;

GADEK, TR ;

HOLM, M ;

ROMAN, R ;

CHAN, HW ;

WENZ, M ;

NORTHROP, JP ;

RINGOLD, GM ;

DANIELSEN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7413-7417

[10] CATIONIC LIPOSOME-MEDIATED TRANSFECTION [J].

FELGNER, PL ;

RINGOLD, GM .

NATURE, 1989, 337 (6205) :387-388

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