High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles

被引：5

作者：

Chen, Ji-Feng ^{[1
,3
,4
]}

Li, Ying ^{[2
]}

Wang, Zhen-Fang ^{[2
]}

Li, Ji-Lun ^{[2
]}

Jiang, Wei ^{[2
]}

Li, Shao-Hua ^{[1
,4
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

[2] China Agr Univ, Dept Microbiol & Immunol, Beijing 100094, Peoples R China

[3] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Peoples R China

[4] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2009年 / 51卷 / 04期

关键词：

bacterial magnetic particle; detection sensitivity; ELISA; fluoroimmunoassay; grapevine fanleaf virus; prunus necrotic ring spot virus; MAGNETOTACTIC BACTERIA; IMMUNOASSAY;

D O I：

10.1111/j.1744-7909.2008.00785.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1 x 10(6) dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (10(6)) higher than an ELISA-based method of detection PNRSV and GFLV.

引用

页码：409 / 413

页数：5

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