Detection and identification of bacteria using direct injection inductively coupled plasma mass spectroscopy

被引:21
作者
Gikunju, CM
Lev, SM [1 ]
Birenzvige, A
Schaefer, DM
机构
[1] Towson Univ, Mat Res Lab, Towson, MD 21252 USA
[2] SBCCOM Edgewood Facil, Aberdeen Proving Ground, MD USA
基金
美国国家科学基金会;
关键词
direct injection ICP-MS; inorganic chemical fingerprinting; bacteria;
D O I
10.1016/j.talanta.2003.09.012
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this study, an inductively coupled plasma mass spectrometer (ICP-MS) was used for the inorganic chemical characterization of biological materials by direct injection. ICP-MS has the advantage of sub-nanogram/gram detection limits for most elements making it a sensitive tool for the detection and characterization of acrosolized biological material. Suspended, microgram-sized samples of Bacillus subtilis spores (BG), Bacillus subtilis vegetative cells (Bg), and Bacillus thuringiensis (Bt) were analyzed via direct injection and they exhibit unique chemical signatures reflecting the processing history of each organism. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:741 / 744
页数:4
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