Development and Implementation of a Molecular Diagnostic Platform for Daily Rapid Detection of 15 Respiratory Viruses

被引：99

作者：

Tivejung-Lindell, Annika ^{[1
,2
]}

Rotzen-Ostlund, Maria ^{[1
,2
]}

Gupta, Shawon ^{[1
,2
]}

Ullstrand, Richard ^{[1
,2
]}

Grillner, Lena ^{[1
,2
]}

Zweygberg-Wirgart, Benita ^{[1
,2
]}

Allander, Tobias ^{[1
,2
]}

机构：

[1] Karolinska Univ Hosp, Dept Clin Microbiol, SE-17176 Stockholm, Sweden

[2] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

来源：

JOURNAL OF MEDICAL VIROLOGY | 2009年 / 81卷 / 01期

基金：

瑞典研究理事会;

关键词：

real-time PCR; respiratory tract infection; influenza; respiratory syncytial virus; human bocavirus; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; INFLUENZA-B VIRUSES; SYNCYTIAL-VIRUS; HUMAN BOCAVIRUS; CEREBROSPINAL-FLUID; CLINICAL SPECIMENS; ASEPTIC-MENINGITIS; ENTEROVIRAL RNA; TRACT INFECTION;

D O I：

10.1002/jmv.21368

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Acute respiratory tract infections are caused by a large number of viruses. Diagnostic methods have until recently been available only for a limited number of these viruses. With the objective to achieve sensitive assays for all respiratory viruses, a rational workflow in the laboratory, and a short turn-around time, a real-time PCR diagnostic platform for daily rapid detection of 15 respiratory viruses was developed. The system was evaluated on 585 stored nasopharyngeal aspirates from hospitalized children. Previous analysis by immunofluorescence and virus isolation identified viruses in 37% of the samples while the new PCR diagnostic panel detected 57% virus positive samples. The new platform was introduced in the laboratory in October 2007 and has then fully replaced the standard immunofluorescence assay for rapid detection of viruses and virus isolation. J. Med. Virol. 81:167-175, 2009. (c) 2008 Wiley-Liss, Inc.

引用

页码：167 / 175

页数：9

共 36 条

[31] Comparison of the NOW Influenza A & B, NOW Flu A, NOW Flu B, and directigen Flu A+B assays, and immunofluorescence with viral culture for the detection of Influenza A and B viruses [J].