Polymorphism of the bm86 gene in South American strains of the cattle tick Boophilus microplus

被引：38

作者：

Sossai, S ^{[1
]}

Peconick, AP ^{[1
]}

Sales-Junior, A ^{[1
]}

Marcelino, FC ^{[1
]}

Vargas, MI ^{[1
]}

Neves, ES ^{[1
]}

Patarroyo, JH ^{[1
]}

机构：

[1] Univ Fed Vicosa, Lab Biol & Control Haematozoa & Vectors, BIOAGRO Vet Dept, BR-36570000 Vicosa, MG, Brazil

来源：

EXPERIMENTAL AND APPLIED ACAROLOGY | 2005年 / 37卷 / 3-4期

关键词：

Boophilus microplus; bm86 and bm95 genes; Bm86 and Bm95 proteins; genetic variability;

D O I：

10.1007/s10493-005-3262-7

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Thirty Boophilus microplus strains from various geographic regions of Brazil, Argentina, Uruguay, Venezuela and Colombia were analyzed for the bm86 and bm95 gene. A fragment of cDNA of 794 base pairs of the parasite larvae, included between nucleotides 278-1071s, was amplified and cloned on the pGEM-T vector. Two random clones were sequenced for each population and the nucleotides 278-1071 and predicted amino acid sequences compared with the bm86 and bm95 genes. Variations from 1.76 to 3.65% were detected in the nucleotides sequence when compared with the homologous sequence of the bm86 gene and a 3.4-6.08% in the homologous amino acid sequence of the Bm86 protein. When the sequences obtained were compared with the bm95 gene, variations from 0.50 to 3.15% were detected. Variations from 1.14 to 4.56% were detected for the Bm95 protein homologous sequences in the deduced amino acid sequence. Only five of the 30 strains analyzed presented two different types of alleles expressed and the two alleles of the Alegre population and allele 1 of the Betim population were the most divergent of all those analyzed.

引用

页码：199 / 214

页数：16

共 27 条

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