Validation of Volume-Pressure Recording Tail-Cuff Blood Pressure Measurements

BACKGROUND The American Heart Association has recommended tail-cuff blood pressure measurement for high-throughput experimental designs, including mutagenesis screens and genetic crosses. However, some tail-cuff methods show good agreement with radiotelemetry and others do not, indicating that each tail-cuff method requires independent validation. METHODS We validated the volume-pressure recording (VPR) tail-cuff method by comparison to simultaneous radiotelemetry measurements. RESULTS Bland-Altman analysis of 560 cycles from 26 independent measurement sessions showed good agreement between, VPR and radiotelemetry measurements, with tail-cuff measurements being 0.25 mm Hg lower than telemetry measurements on average. However, the VPR method was less accurate, compared to radiotelemetry, at extreme high and low (i.e., <110 or >180 mm Hg) systolic blood pressures (SBPs). CONCLUSIONS We conclude that the VPR tail-cuff method provides accurate blood pressure measurements over the physiological range of blood pressure in mice.