Simple bioseparations using self-cleaving elastin-like polypeptide tags

被引：189

作者：

Banki, MR

Feng, LA

Wood, DW

机构：

[1] Princeton Univ, Dept Chem Engn, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

来源：

NATURE METHODS | 2005年 / 2卷 / 09期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/nmeth787

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce a new method for the purification of recombinant proteins expressed in Escherichia coli using self-cleaving elastin-like polypeptide (ELP) fusion tags without the need for affinity chromatography or proteolytic tag removal. Using this method we obtained high purity, activity and reasonable yields for ten diverse target proteins.

引用

收藏

页码：659 / 661

页数：3

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