Enhanced uptake of nanoparticle drug carriers via a thermoresponsive shell enhances cytotoxicity in a cancer cell line

被引：66

作者：

Abulateefeh, Samer R. ^{[1
,2
]}

Spain, Sebastian G. ^{[1
]}

Thurecht, Kristofer J. ^{[3
,4
]}

Aylott, Jonathan W. ^{[1
]}

Chan, Weng C. ^{[1
]}

Garnett, Martin C. ^{[1
]}

Alexander, Cameron ^{[1
]}

机构：

[1] Univ Nottingham, Sch Pharm, Nottingham NG7 2RD, England

[2] Univ Jordan, Fac Pharm, Dept Pharmaceut & Pharmaceut Technol, Amman 11942, Jordan

[3] Univ Queensland, Australian Inst Bioengn & Nanotechnol, St Lucia, Qld 4072, Australia

[4] Univ Queensland, Ctr Adv Imaging, St Lucia, Qld 4072, Australia

来源：

BIOMATERIALS SCIENCE | 2013年 / 1卷 / 04期

基金：

澳大利亚研究理事会; 英国工程与自然科学研究理事会;

关键词：

PLGA NANOPARTICLES; IN-VITRO; POLYPEPTIDE NANOPARTICLES; GOLD NANOPARTICLES; DELIVERY; HYPERTHERMIA; PACLITAXEL; VIVO; NANOPRECIPITATION; TEMPERATURE;

D O I：

10.1039/c2bm00184e

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

082905 [生物质能源与材料]; 100103 [病原生物学];

摘要：

Polymer particles consisting of a biodegradable poly[lactide-co-glycolide] (PLGA) core and a thermoresponsive shell have been formulated to encapsulate the dye rhodamine 6G and the potent cytotoxic drug paclitaxel. Cellular uptake of these particles is significantly enhanced above the thermal transition temperature (TTT) of the polymer shells in the human breast carcinoma cell line MCF-7 as determined by flow cytometry and fluorescence microscopy. Paclitaxel-loaded particles display reduced and enhanced cytotoxicity below and above the TTT respectively compared to unencapsulated drug. The data suggests a potential route to enhanced anti-cancer efficacy through temperature-mediated cell targeting.

引用

页码：434 / 442

页数：9

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