Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma

被引：231

作者：

Bo, Hao ^{[1
,2
,3
,4
,5
]}

Gong, Zhaojian ^{[3
,4
,6
]}

Zhang, Wenling ^{[3
,4
]}

Li, Xiayu ^{[5
]}

Zeng, Yong ^{[1
,2
]}

Liao, Qianjin ^{[1
,2
]}

Chen, Pan ^{[1
,2
]}

Shi, Lei ^{[6
]}

Lian, Yu ^{[3
,4
]}

Jing, Yizhou ^{[3
,4
]}

Tang, Ke ^{[3
,4
]}

Li, Zheng ^{[1
,2
,3
,4
,5
]}

Zhou, Yanhong ^{[1
,2
,3
,4
,5
]}

Zhou, Ming ^{[1
,2
,3
,4
,5
]}

Xiang, Bo ^{[1
,2
,3
,4
,5
]}

Li, Xiaoling ^{[1
,2
,3
,4
,5
]}

Yang, Jianbo ^{[1
,2
,7
,8
]}

Xiong, Wei ^{[1
,2
,3
,4
,5
]}

Li, Guiyuan ^{[1
,2
,3
,4
,5
]}

Zeng, Zhaoyang ^{[1
,2
,3
,4
,5
]}

机构：

[1] Cent South Univ, Hunan Canc Hosp, Changsha, Hunan, Peoples R China

[2] Cent South Univ, Affiliated Canc Hosp, Xiangya Sch Med, Changsha, Hunan, Peoples R China

[3] Cent South Univ, Chinese Minist Hlth, Key Lab Carcinogenesis, Changsha, Hunan, Peoples R China

[4] Cent South Univ, Chinese Minist Educ, Key Lab Carcinogenesis & Canc Invas, Canc Res Inst, Changsha, Hunan, Peoples R China

[5] Cent South Univ, Xiangya Hosp 3, Hunan Key Lab Nonresolving Inflammat & Canc, Dis Genome Res Ctr, Changsha, Hunan, Peoples R China

[6] Cent South Univ, Xiangya Hosp 2, Dept Oral & Maxillofacial Surg, Changsha, Hunan, Peoples R China

[7] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA

[8] Univ Minnesota, Masonic Canc Ctr, Minneapolis, MN USA

来源：

ONCOTARGET | 2015年 / 6卷 / 24期

基金：

中国国家自然科学基金;

关键词：

long non-coding RNA (LncRNA); AFAP1 antisense RNA1 (AFAP1-AS1); nasopharyngeal carcinoma (NPC); metastasis; prognosis; GENE-EXPRESSION; CELL-PROLIFERATION; CHROMOSOME; 3P21; LABEL-FREE; PROTEIN; LNCRNA; METASTASIS; AFAP-110; OVEREXPRESSION; CARCINOGENESIS;

D O I：

10.18632/oncotarget.4057

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Altered expression of long noncoding RNAs (lncRNAs) associated with human carcinogenesis. We performed a cDNA microarray analysis of lncRNA expression in 12 cases of nasopharyngeal carcinoma (NPC) and 4 non-tumor nasopharyngeal epitheliums. One lncRNA, actin filament associated protein 1 antisense RNA1 (AFAP1-AS1), was identified and selected for further study. AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability. AFAP1-AS1 knockdown also increased AFAP1 protein expression. Proteomic and bioinformatics analyses suggested that AFAP1-AS1 affected the expression of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. AFAP1-AS1 might be a potential novel marker that can predict cancer patient prognosis and as a potential therapeutic target for NPC.

引用

页码：20404 / 20418

页数：15

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