The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer

被引：603

作者：

Chakravarty, Dimple ^{[1
,2
,3
]}

Sboner, Andrea ^{[1
,2
,3
,4
]}

Nair, Sujit S. ^{[5
]}

Giannopoulou, Eugenia ^{[6
,7
,8
]}

Li, Ruohan ^{[9
]}

Hennig, Sven ^{[10
]}

Mosquera, Juan Miguel ^{[1
,2
,3
]}

Pauwels, Jonathan

Park, Kyung

Kossai, Myriam ^{[1
,2
,3
]}

MacDonald, Theresa Y.

Fontugne, Jacqueline ^{[1
,2
,3
]}

Erho, Nicholas ^{[11
]}

Vergara, Ismael A. ^{[11
]}

Ghadessi, Mercedeh ^{[11
]}

Davicioni, Elai ^{[11
]}

Jenkins, Robert B. ^{[12
]}

Palanisamy, Nallasivam ^{[13
,14
]}

Chen, Zhengming ^{[15
]}

Nakagawa, Shinichi ^{[16
]}

Hirose, Tetsuro ^{[17
]}

Bander, Neil H. ^{[18
]}

Beltran, Himisha ^{[1
,2
,3
]}

Fox, Archa H. ^{[9
]}

Elemento, Olivier ^{[1
,2
,3
,4
]}

Rubin, Mark A. ^{[1
,2
,3
]}

机构：

[1] Cornell Univ, Weill Med Coll, Dept Pathol & Lab Med, 413 East 69th St,Room 1402, New York, NY 10021 USA

[2] Cornell Univ, Weill Med Coll, Inst Precis Med, New York, NY 10021 USA

[3] New York Presbyterian Hosp, New York, NY 10021 USA

[4] Cornell Univ, Weill Cornell Med Coll, Inst Computat Biomed, New York, NY 10021 USA

[5] George Washington Univ, Sch Med & Hlth Sci, Dept Biochem & Mol Med, Washington, DC 20037 USA

[6] CUNY, New York City Coll Technol, Dept Biol Sci, Brooklyn, NY 11201 USA

[7] Hosp Special Surg, Arthrit & Tissue Degenerat Program, New York, NY 10021 USA

[8] Hosp Special Surg, David Z Rosensweig Genom Res Ctr, New York, NY 10021 USA

[9] Univ Western Australia, Sch Biomed Biomol & Chem Sci, Crawley, WA 6009, Australia

[10] Chem Genom Ctr, D-44227 Dortmund, Germany

[11] GenomeDx Biosci, Res & Dev, Vancouver, BC V6B 1B8, Canada

[12] Mayo Clin, Dept Pathol & Lab Med, Rochester, MN 55905 USA

[13] Univ Michigan, Michigan Ctr Translat Pathol, Ann Arbor, MI 48105 USA

[14] Henry Ford Hlth Syst, Med Grp Urol Prostate Canc Res, Detroit, MI 48202 USA

[15] Weill Cornell Med Coll, Dept Publ Hlth, Div Biostat & Epidemiol, New York, NY 10021 USA

[16] RIKEN, Adv Res Inst, RNA Biol Lab, Wako, Saitama 3510198, Japan

[17] Hokkaido Univ, Inst Med Genet, Kita Ku, Sapporo, Hokkaido 0600815, Japan

[18] Cornell Univ, Weill Cornell Med Coll, Dept Urol, New York, NY 10021 USA

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

LONG NONCODING RNA; GENE-EXPRESSION PROFILES; ANDROGEN RECEPTOR; MEMBRANE ANTIGEN; RADICAL PROSTATECTOMY; INCREASED SURVIVAL; NUCLEAR-BODIES; PROGRESSION; CHROMATIN; REVEALS;

D O I：

10.1038/ncomms6383

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The androgen receptor (AR) plays a central role in establishing an oncogenic cascade that drives prostate cancer progression. Some prostate cancers escape androgen dependence and are often associated with an aggressive phenotype. The oestrogen receptor alpha (ER alpha) is expressed in prostate cancers, independent of AR status. However, the role of ER alpha remains elusive. Using a combination of chromatin immunoprecipitation (ChIP) and RNA-sequencing data, we identified an ER alpha-specific non-coding transcriptome signature. Among putatively ER alpha-regulated intergenic long non-coding RNAs (lncRNAs), we identified nuclear enriched abundant transcript 1 (NEAT1) as the most significantly overexpressed lncRNA in prostate cancer. Analysis of two large clinical cohorts also revealed that NEAT1 expression is associated with prostate cancer progression. Prostate cancer cells expressing high levels of NEAT1 were recalcitrant to androgen or AR antagonists. Finally, we provide evidence that NEAT1 drives oncogenic growth by altering the epigenetic landscape of target gene promoters to favour transcription.

引用

页数：16

共 58 条

[1]

lncRNAdb: a reference database for long noncoding RNAs [J].

Amaral, Paulo P. ;

Clark, Michael B. ;

Gascoigne, Dennis K. ;

Dinger, Marcel E. ;

Mattick, John S. .

NUCLEIC ACIDS RESEARCH, 2011, 39 :D146-D151

[2]

Identification of the Transcription Factor Single-Minded Homologue 2 as a Potential Biomarker and Immunotherapy Target in Prostate Cancer [J].

Arredouani, Mohamed S. ;

Lu, Bin ;

Bhasin, Manoj ;

Eljanne, Miriam ;

Yue, Wen ;

Mosquera, Juan-Miguel ;

Bubley, Glenn J. ;

Li, Vivian ;

Rubin, Mark A. ;

Libermann, Towia A. ;

Sanda, Martin G. .

CLINICAL CANCER RESEARCH, 2009, 15 (18) :5794-5802

[3]

Exome sequencing identifies recurrent SPOP, FOXA1 and MED12 mutations in prostate cancer [J].

Barbieri, Christopher E. ;

Baca, Sylvan C. ;

Lawrence, Michael S. ;

Demichelis, Francesca ;

Blattner, Mirjam ;

Theurillat, Jean-Philippe ;

White, Thomas A. ;

Stojanov, Petar ;

Van Allen, Eliezer ;

Stransky, Nicolas ;

Nickerson, Elizabeth ;

Chae, Sung-Suk ;

Boysen, Gunther ;

Auclair, Daniel ;

Onofrio, Robert C. ;

Park, Kyung ;

Kitabayashi, Naoki ;

MacDonald, Theresa Y. ;

Sheikh, Karen ;

Vuong, Terry ;

Guiducci, Candace ;

Cibulskis, Kristian ;

Sivachenko, Andrey ;

Carter, Scott L. ;

Saksena, Gordon ;

Voet, Douglas ;

Hussain, Wasay M. ;

Ramos, Alex H. ;

Winckler, Wendy ;

Redman, Michelle C. ;

Ardlie, Kristin ;

Tewari, Ashutosh K. ;

Mosquera, Juan Miguel ;

Rupp, Niels ;

Wild, Peter J. ;

Moch, Holger ;

Morrissey, Colm ;

Nelson, Peter S. ;

Kantoff, Philip W. ;

Gabriel, Stacey B. ;

Golub, Todd R. ;

Meyerson, Matthew ;

Lander, Eric S. ;

Getz, Gad ;

Rubin, Mark A. ;

Garraway, Levi A. .

NATURE GENETICS, 2012, 44 (06) :685-U107

[4]

Prostate cancer genes associated with TMPRSS2-ERG gene fusion and prognostic of biochemical recurrence in multiple cohorts [J].

Barwick, B. G. ;

Abramovitz, M. ;

Kodani, M. ;

Moreno, C. S. ;

Nam, R. ;

Tang, W. ;

Bouzyk, M. ;

Seth, A. ;

Leyland-Jones, B. .

BRITISH JOURNAL OF CANCER, 2010, 102 (03) :570-576

[5]

CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[6]

RNA meets chromatin [J].

Bernstein, E ;

Allis, CD .

GENES & DEVELOPMENT, 2005, 19 (14) :1635-1655

[7]

Molecular alterations in primary prostate cancer after androgen ablation therapy [J].

Best, CJM ;

Gillespie, JW ;

Yi, YJ ;

Chandramouli, GVR ;

Perlmutter, MA ;

Gathright, Y ;

Erickson, HS ;

Georgevich, L ;

Tangrea, MA ;

Duray, PH ;

González, S ;

Velasco, A ;

Linehan, WM ;

Matusik, RJ ;

Price, DK ;

Figg, WD ;

Emmert-Buck, MR ;

Chuaqui, RF .

CLINICAL CANCER RESEARCH, 2005, 11 (19) :6823-6834

[8]

Use of Gleason score, prostate specific antigen, seminal vesicle and margin status to predict biochemical failure after radical prostatectomy [J].

Blute, ML ;

Bergstralh, EJ ;

Iocca, A ;

Scherer, B ;

Zincke, H .

JOURNAL OF UROLOGY, 2001, 165 (01) :119-125

[9]

Paraspeckles: nuclear bodies built on long noncoding RNA [J].

Bond, Charles S. ;

Fox, Archa H. .

JOURNAL OF CELL BIOLOGY, 2009, 186 (05) :637-644

[10]

Estrogen receptor expression in prostate cancer and premalignant prostatic lesions [J].

Bonkhoff, H ;

Fixemer, T ;

Hunsicker, I ;

Remberger, K .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (02) :641-647

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