Label-free electrochemical detection of protein based on a ferrocene-bearing cationic polythiophene and aptamer

被引：151

作者：

Le Floch, Fabien ^{[1
]}

Ho, Hoang A. ^{[1
]}

Leclerc, Mario ^{[1
]}

机构：

[1] Univ Laval, Dept Chem, Quebec City, PQ G1K 7P4, Canada

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 13期

关键词：

D O I：

10.1021/ac0521955

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two label-free electrochemical methods for the detection of human alpha-thrombin using a water-soluble, ferrocene-functionalized polythiophene transducer and a single-stranded oligonucleotide aptamer probe are described. The first approach is a direct method in which the recorded current decreases upon addition of the targeted protein. The second one requires more steps and the additional utilization of PNA probes and nuclease enzyme. This indirect method leads to an increase of the electrical signal as a function of the concentration of human alpha-thrombin with a detection limit of 75 fmol.

引用

页码：4727 / 4731

页数：5