Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor

被引：667

作者：

Xiao, Y

Lubin, AA

Heeger, AJ

Plaxco, KW ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

[5] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 34期

关键词：

aptamers; biosensors; DNA structures; electron transfer; thrombin;

D O I：

10.1002/anie.200500989

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin-binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5456 / 5459

页数：4

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