Lipase immobilisation on to polymeric membranes

被引：35

作者：

Carneiro-da-Cunha, MG

Rocha, JMS

Garcia, FAP

Gil, MH ^{[1
]}

机构：

[1] Univ Coimbra, Dept Chem Engn, P-3000 Coimbra, Portugal

[2] Univ Fed Pernambuco, LIKA, Dept Biochem, Recife, PE, Brazil

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 06期

关键词：

immobilisation; lipase; membrane;

D O I：

10.1023/A:1008913824571

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipase (EC 3.1.1.3) from Candida rugosa was covalently immobilised on to cellulose, cellulose derivatives (cellulose acetate and cellulose phthalate) and cellulose composite membranes using activating agents such as sodium periodate or carbodiimide. Other non-cellulosic polymeric membranes (nylon, polyurethane, chitosan and hydroxyethyl methacrylate-co-methyl methacrylate) were also prepared and used for lipase immobilisation. The results obtained showed that the expressed activities are of the same order of magnitude for similar enzyme loadings when compared with those obtained from literature.

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收藏

页码：403 / 409

页数：7

相关论文

共 15 条

[1] The performance of enzyme-membrane reactors with immobilized lipase [J].

Bouwer, ST ;

Cuperus, FP ;

Derksen, JTP .

ENZYME AND MICROBIAL TECHNOLOGY, 1997, 21 (04) :291-296

[2] Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene [J].

Dharmsthiti, S ;

Luchai, S .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1998, 86 (03) :335-337

[3] BIOCHEMICAL-ENGINEERING OF BIOCATALYSTS IMMOBILIZED ON CELLULOSIC MATERIALS [J].

GEMEINER, P ;

STEFUCA, V ;

BALES, V .

ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (07) :551-566

[4] COVALENT BINDING OF UREASE ON AMMONIUM-SELECTIVE POTENTIOMETRIC MEMBRANES [J].

GIL, MH ;

PIEDADE, AP ;

ALEGRET, S ;

ALONSO, J ;

MARTINEZFABREGAS, E ;

ORELLANA, A .

BIOSENSORS & BIOELECTRONICS, 1992, 7 (09) :645-652

[5] LIPASE KINETICS - HYDROLYSIS OF TRIACETIN BY LIPASE FROM CANDIDA-CYLINDRACEA IN A HOLLOW-FIBER MEMBRANE REACTOR [J].

GUIT, RPM ;

KLOOSTERMAN, M ;

MEINDERSMA, GW ;

MAYER, M ;

MEIJER, EM .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (07) :727-732

[6] Alcoholysis and glyceride synthesis with immobilized lipase on controlled-pore glass of varying hydrophobicity in supercritical carbon dioxide [J].

Gunnlaugsdottir, H ;

Wannerberger, K ;

Sivik, B .

ENZYME AND MICROBIAL TECHNOLOGY, 1998, 22 (05) :360-367

[7] BIOREACTOR FOR ENZYMATIC-REACTION OF FAT AND FATTY-ACID DERIVATIVES .3. CONTINUOUS HYDROLYSIS OF OLIVE OIL BY LIPASE IN MICROPOROUS HYDROPHOBIC MEMBRANE BIOREACTOR [J].

HOQ, MM ;

YAMANE, T ;

SHIMIZU, S ;

FUNADA, T ;

ISHIDA, S .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1985, 62 (06) :1016-1021

[8] Immobilization of lipase onto lipophilic polymer particles and application to oil hydrolysis [J].

Murray, M ;

Rooney, D ;

VanNeikerk, M ;

Montenegro, A ;

Weatherley, LR .

PROCESS BIOCHEMISTRY, 1997, 32 (06) :479-486

[9] A NEW ASSAY OF MICROBIAL LIPASES WITH EMULSIFIED TRIOLEOYL GLYCEROL [J].

PELED, N ;

KRENZ, MC .

ANALYTICAL BIOCHEMISTRY, 1981, 112 (02) :219-222

[10] Characterization of cellulose derivatives - Relevance to sensor development [J].

Piedade, AP ;

Guthrie, JT ;

Kazlauciunas, A ;

Gil, MH .

CELLULOSE, 1995, 2 (04) :243-263