Surfection: a new platform for transfected cell arrays

被引：32

作者：

Chang, FH ^{[1
]}

Lee, CH

Chen, MT

Kuo, CC

Chiang, YL

Hang, CY

Roffler, S

机构：

[1] Natl Taiwan Univ, Coll Med, Inst Biochem & Mol Biol, Taipei, Taiwan

[2] Natl Hlth Res Inst, Div Mol & Gen Med, Taipei, Taiwan

[3] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 03期

关键词：

D O I：

10.1093/nar/gnh029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid-cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface-mediated transfection method, termed 'surfection', can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening.

引用

页数：11

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