Surfection: a new platform for transfected cell arrays

被引:32
作者
Chang, FH [1 ]
Lee, CH
Chen, MT
Kuo, CC
Chiang, YL
Hang, CY
Roffler, S
机构
[1] Natl Taiwan Univ, Coll Med, Inst Biochem & Mol Biol, Taipei, Taiwan
[2] Natl Hlth Res Inst, Div Mol & Gen Med, Taipei, Taiwan
[3] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan
关键词
D O I
10.1093/nar/gnh029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid-cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface-mediated transfection method, termed 'surfection', can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening.
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页数:11
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