Comparison of immunochromatography, PCR and culture methods for the detection of Campylobacter bacteria

被引：6

作者：

Dey, Shuvra Kanti ^{[1
]}

Nishimura, Shuichi ^{[2
]}

Okitsu, Shoko

Hayakawa, Satoshi

Mizuguchi, Masashi ^{[4
]}

Ushijima, Hiroshi ^{[3
]}

机构：

[1] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA

[2] Nishimura Clin, Maizuru, Kyoto, Japan

[3] Nihon Univ, Dept Pathol & Microbiol, Div Microbiol, Itabashi Ku, Tokyo 1738610, Japan

[4] Univ Tokyo, Grad Sch Med, Dept Dev Med Sci, Tokyo, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 91卷 / 03期

关键词：

Immunoenzymatic; Rapid detection; PCR; Culture methods; Campylobacter jejuni; MULTIPLEX PCR; JEJUNI; COLI; IDENTIFICATION; HYBRIDIZATION; INFECTIONS; DIAGNOSIS; FETUS; ASSAY;

D O I：

10.1016/j.mimet.2012.09.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A total of 463 fecal specimens from human patients were tested for the presence of Camphylobacter by a commercial immunochromatography kit (ImmunoCard STAT! CAMPY), polymerase chain reaction (PCR) and conventional bacteriological (CB) methods. Using culture as the standard of reference, the ImmunoCard STAT! CAMPY assay had a sensitivity of 86% and a specificity of 100%. On the other hand, using PCR as the standard of reference, ImmunoCard STAT! CAMPY assay had a sensitivity of 90.5% and a specificity of 100%. (c) 2012 Published by Elsevier B.V.

引用

页码：566 / 568

页数：3