Human cardiomyocyte calcium handling and transverse tubules in mid-stage of post-myocardial-infarction heart failure

被引：50

作者：

Hoydal, Morten Andre ^{[1
,3
]}

Kirkeby-Garstad, Idar ^{[2
,3
]}

Karevold, Asbjorn ^{[2
,3
]}

Wiseth, Rune ^{[2
,3
]}

Haaverstad, Rune ^{[3
]}

Wahba, Alexander ^{[1
,2
,3
]}

Stolen, Tomas L. ^{[1
,3
]}

Contu, Riccardo ^{[4
]}

Condorelli, Gianluigi ^{[4
]}

Ellingsen, Oyvind ^{[1
,2
,3
]}

Smith, Godfrey L. ^{[2
,5
,6
]}

Kemi, Ole J. ^{[5
,6
]}

Wisloff, Ulrik ^{[1
,2
,7
]}

机构：

[1] Norwegian Univ Sci & Technol NTNU, Fac Med & Hlth, Dept Circulat & Med Imaging, Trondheim, Norway

[2] Norwegian Univ Sci & Technol NTNU, KG Jebsen Ctr Exercise Med, Trondheim, Norway

[3] St Olavs Univ Hosp, Trondheim, Norway

[4] Humanitas Univ, CNR Natl Res Council Italy, Humanitas Res Hosp, Dept Cardiovasc Med, Milan, Italy

[5] Univ Glasgow, Coll Med Vet & Life Sci, Inst Cardiovasc & Med Sci, Glasgow, Lanark, Scotland

[6] Univ Glasgow, Coll Med Vet & Life Sci, Sch Life Sci, Glasgow, Lanark, Scotland

[7] Univ Queensland, Sch Human Movement & Nutr Sci, Brisbane, Qld, Australia

来源：

ESC HEART FAILURE | 2018年 / 5卷 / 03期

关键词：

Heart failure; Myocardial infarction; Calcium handling; Cardiomyocytes; Excitation contraction coupling; SERCA2a; CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE; RYANODINE RECEPTOR PHOSPHORYLATION; NONFAILING HUMAN MYOCARDIUM; FAILING HUMAN MYOCARDIUM; SARCOPLASMIC-RETICULUM; DILATED CARDIOMYOPATHY; REDUCED SYNCHRONY; CA2+ RELEASE; T-TUBULES; VENTRICULAR MYOCYTES;

D O I：

10.1002/ehf2.12271

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

AimsCellular processes in the heart rely mainly on studies from experimental animal models or explanted hearts from patients with terminal end-stage heart failure (HF). To address this limitation, we provide data on excitation contraction coupling, cardiomyocyte contraction and relaxation, and Ca2+ handling in post-myocardial-infarction (MI) patients at mid-stage of HF. Methods and resultsNine MI patients and eight control patients without MI (non-MI) were included. Biopsies were taken from the left ventricular myocardium and processed for further measurements with epifluorescence and confocal microscopy. Cardiomyocyte function was progressively impaired in MI cardiomyocytes compared with non-MI cardiomyocytes when increasing electrical stimulation towards frequencies that simulate heart rates during physical activity (2Hz); at 3Hz, we observed almost total breakdown of function in MI. Concurrently, we observed impaired Ca2+ handling with more spontaneous Ca2+ release events, increased diastolic Ca2+, lower Ca2+ amplitude, and prolonged time to diastolic Ca2+ removal in MI (P<0.01). Significantly reduced transverse-tubule density (-35%, P<0.01) and sarcoplasmic reticulum Ca2+ adenosine triphosphatase 2a (SERCA2a) function (-26%, P<0.01) in MI cardiomyocytes may explain the findings. Reduced protein phosphorylation of phospholamban (PLB) serine-16 and threonine-17 in MI provides further mechanisms to the reduced function. ConclusionsDepressed cardiomyocyte contraction and relaxation were associated with impaired intracellular Ca2+ handling due to impaired SERCA2a activity caused by a combination of alteration in the PLB/SERCA2a ratio and chronic dephosphorylation of PLB as well as loss of transverse tubules, which disrupts normal intracellular Ca2+ homeostasis and handling. This is the first study that presents these mechanisms from viable and intact cardiomyocytes isolated from the left ventricle of human hearts at mid-stage of post-MI HF.

引用

页码：332 / 342

页数：11

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