Polymorphic Fibrillation of the Destabilized Fourth Fasciclin-1 Domain Mutant A546T of the Transforming Growth Factor-β-induced Protein (TGFBIp) Occurs through Multiple Pathways with Different Oligomeric Intermediates

被引:19
作者
Andreasen, Maria [1 ,2 ]
Nielsen, Soren B. [1 ,2 ]
Runager, Kasper [1 ,2 ]
Christianse, Gunna [3 ]
Nielse, Niels Chr. [4 ,5 ]
Enghild, Jan J. [1 ,2 ]
Otzen, Daniel E. [1 ,2 ]
机构
[1] Aarhus Univ, Ctr Insoluble Prot Struct inSPIN, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus, Denmark
[2] Aarhus Univ, Dept Mol Biol & Genet, DK-8000 Aarhus, Denmark
[3] Aarhus Univ, Dept Biomed, DK-8000 Aarhus, Denmark
[4] Aarhus Univ, inSPIN, iNANO, DK-8000 Aarhus, Denmark
[5] Aarhus Univ, Dept Chem, DK-8000 Aarhus, Denmark
基金
新加坡国家研究基金会;
关键词
FIELD-FLOW FRACTIONATION; KERATO-EPITHELIN MUTATIONS; HEAT-INDUCED AGGREGATION; CORNEAL DYSTROPHIES; VESICLE PERMEABILIZATION; SEQUENCE-ANALYSIS; LACTOGLOBULIN AB; GENE-MUTATIONS; BETA-IG-H3; PH;
D O I
10.1074/jbc.M112.379552
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mutations in the transforming growth factor beta-induced protein (TGFBIp) are linked to the development of corneal dystrophies in which abnormal protein deposition in the cornea leads to a loss of corneal transparency and ultimately blindness. Different mutations give rise to phenotypically distinct corneal dystrophies. Most mutations are located in the fourth fasciclin-1 domain (FAS1-4). The amino acid substitution A546T in the FAS1-4 domain is linked to the development of lattice corneal dystrophy with amyloid deposits in the superficial and deep stroma, classifying it as an amyloid disease. Here we provide a detailed description of the fibrillation of the isolated FAS1-4 domain carrying the A546T substitution. The A546T substitution leads to a significant destabilization of FAS1-4 and induces a partially folded structure with increased surface exposure of hydrophobic patches. The mutation also leads to two distinct fibril morphologies. Long straight fibrils composed of pure beta-sheet structure are formed at lower concentrations, whereas short and curly fibrils containing a mixture of alpha-helical and beta-sheet structures are formed at higher concentrations. The formation of short and curly fibrils is preceded by the formation of a small number of oligomeric species with high membrane permeabilization potential and rapid fibril formation. The long straight fibrils are formed more slowly and through progressively bigger oligomers that lose their membrane permeabilization potential as fibrillation proceeds beyond the lag phase. These different fibril classes and associated biochemical differences may lead to different clinical symptoms associated with the mutation.
引用
收藏
页码:34730 / 34742
页数:13
相关论文
共 68 条
[61]  
Sokolov Yuri, 2006, J Gen Physiol, V128, P637, DOI 10.1085/jgp.200609533
[62]  
Streeten BW, 1999, ARCH OPHTHALMOL-CHIC, V117, P67
[63]   Vesicle permeabilization by protofibrillar α-synuclein is sensitive to Parkinson's disease-linked mutations and occurs by a pore-like mechanism [J].
Volles, MJ ;
Lansbury, PT .
BIOCHEMISTRY, 2002, 41 (14) :4595-4602
[64]   PROPERTIES OF AN ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION CHANNEL HAVING ONE PERMEABLE WALL [J].
WAHLUND, KG ;
GIDDINGS, JC .
ANALYTICAL CHEMISTRY, 1987, 59 (09) :1332-1339
[65]  
Weiss JS, 2008, CORNEA, V27, pS1, DOI 10.1097/ICO.0b013e31817780fb
[66]   Seeding-dependent propagation and maturation of amyloid fibril conformation [J].
Yamaguchi, K ;
Takahashi, S ;
Kawai, T ;
Naiki, H ;
Goto, Y .
JOURNAL OF MOLECULAR BIOLOGY, 2005, 352 (04) :952-960
[67]   FTIR reveals structural differences between native β-sheet proteins and amyloid fibrils [J].
Zandomeneghi, G ;
Krebs, MRH ;
Mccammon, MG ;
Fändrich, M .
PROTEIN SCIENCE, 2004, 13 (12) :3314-3321
[68]   SEQUENCE-ANALYSIS AND NEURONAL EXPRESSION OF FASCICLIN-I IN GRASSHOPPER AND DROSOPHILA [J].
ZINN, K ;
MCALLISTER, L ;
GOODMAN, CS .
CELL, 1988, 53 (04) :577-587