Serotype identification of group B streptococci by PCR and sequencing

被引:118
作者
Kong, FR
Gowan, S
Martin, D
James, G
Gilbert, GL
机构
[1] Westmead Hosp, Inst Clin Pathol & Med Res, Ctr Infect Dis, Westmead, NSW 2145, Australia
[2] Westmead Hosp, Inst Clin Pathol & Med Res, Microbiol Lab Serv, Westmead, NSW 2145, Australia
[3] Inst Environm Sci & Res, Wellington, New Zealand
关键词
D O I
10.1128/JCM.40.1.216-226.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution will be needed to guide the development and use of GBS conjugate vaccines. We designed sequencing primers based on the previously published sequences of the capsular polysaccharide (cps) gene clusters to further define partial cps gene clusters for eight of the nine GBS serotypes (serotypes la to VII). Subsequently, we designed and evaluated primers to identify serotypes Ia, Ib, III, IV, V, and VI directly by PCR and all eight serotypes (serotypes la to VII) by sequence heterogeneity. A total of 206 clinical GBS isolates were used to compare our molecular serotype (MS) identification method with conventional serotyping (CS). All clinical isolates were assigned an MS, whereas 188 of 206 (91.3%) were assigned a serotype by use of antisera, A small number of isolates (serosubtypes III-3 and III-4) showed different serotype specificities between PCR and sequencing, but the PCR results correlated with those obtained by CS. The overall agreement between the MS identification method and CS for isolates for which results of both tests were available was 100% (188 of 188 isolates). The MS identification method is a specific and practical alternative to conventional GBS serotyping and will facilitate epidemiological studies.
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页码:216 / 226
页数:11
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