A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus

被引：99

作者：

Chen, Peng R. ^{[1
]}

Nishida, Satoshi ^{[1
]}

Poor, Catherine B. ^{[1
]}

Cheng, Alice ^{[2
]}

Bae, Taeok ^{[2
,3
]}

Kuechenmeister, Lisa ^{[4
]}

Dunman, Paul M. ^{[4
]}

Missiakas, Dominique ^{[2
]}

He, Chuan ^{[1
]}

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[2] Univ Chicago, Dept Microbiol, Chicago, IL 60637 USA

[3] Indiana Univ NW, Sch Med, Dept Microbiol & Immunol, Gary, IN 46408 USA

[4] 986495 Nebraska Med Ctr, Omaha, NE 68198 USA

来源：

MOLECULAR MICROBIOLOGY | 2009年 / 71卷 / 01期

关键词：

PYRUVATE FORMATE-LYASE; CYSTEINE-SULFENIC ACID; GENE-EXPRESSION; BACILLUS-SUBTILIS; TRANSCRIPTION FACTOR; HYDROGEN-PEROXIDE; VIRULENCE GENES; ORGANIC HYDROPEROXIDES; TOXICOGENOMIC RESPONSE; SIGNAL-TRANSDUCTION;

D O I：

10.1111/j.1365-2958.2008.06518.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress serves as an important host/environmental signal that triggers a wide range of responses from the human pathogen Staphylococcus aureus. Among these, a thiol-based oxidation sensing pathway through a global regulator MgrA controls the virulence and antibiotic resistance of the bacterium. Herein, we report a new thiol-based oxidation sensing and regulation system that is mediated through a parallel global regulator SarZ. SarZ is a functional homologue of MgrA and is shown to affect the expression of similar to 87 genes in S. aureus. It uses a key Cys residue, Cys-13, to sense oxidative stress and to co-ordinate the expression of genes involved in metabolic switching, antibiotic resistance, peroxide stress defence, virulence, and cell wall properties. The discovery of this SarZ-mediated regulation, mostly independent from the MgrA-based regulation, fills a missing gap of oxidation sensing and response in S. aureus.

引用

页码：198 / 211

页数：14

共 79 条

[71] Conservation and diversity of the cellular disulfide bond formation pathways [J].