Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action

被引：17

作者：

Leeper, TC ^{[1
]}

Martin, MB ^{[1
]}

Kim, H ^{[1
]}

Cox, S ^{[1
]}

Semenchenko, V ^{[1
]}

Schmidt, FJ ^{[1
]}

Van Doren, SR ^{[1
]}

机构：

[1] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2002年 / 9卷 / 05期

基金：

美国国家科学基金会; 美国国家航空航天局;

关键词：

D O I：

10.1038/nsb775

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA are thought to form a structural truss to support RNA folding and activity. We determined the structure of this element by NMR and refined the structure using residual dipolar couplings from a sample weakly oriented in a dilute liquid crystalline mixture of polyethylene glycol and hexanol. Dipolar coupling refinement improved the global precision of the structure from 1.5 to 1.2 Angstrom ( to the mean), revised the bend angle between segments of the P5.1 stem and corroborated the structure of the loop region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on opposite sides of the loop, distinguishing it from GNRA tetraloops. The unusual conformation of the juxtaposed uracil residues within the hexaloop may explain their requirement in transactivation assays.

引用

页码：397 / 403

页数：7

共 51 条

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