Active DNA Demethylation in Plant Companion Cells Reinforces Transposon Methylation in Gametes

被引：383

作者：

Ibarra, Christian A. ^{[1
]}

Feng, Xiaoqi ^{[1
]}

Schoft, Vera K. ^{[2
]}

Hsieh, Tzung-Fu ^{[1
]}

Uzawa, Rie ^{[1
]}

Rodrigues, Jessica A. ^{[1
]}

Zemach, Assaf ^{[1
]}

Chumak, Nina ^{[2
]}

Machlicova, Adriana ^{[2
]}

Nishimura, Toshiro ^{[1
]}

Rojas, Denisse ^{[1
]}

Fischer, Robert L. ^{[1
]}

Tamaru, Hisashi ^{[2
]}

Zilberman, Daniel ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[2] Austrian Acad Sci, Gregor Mendel Inst, A-1030 Vienna, Austria

来源：

SCIENCE | 2012年 / 337卷 / 6100期

基金：

奥地利科学基金会; 美国国家科学基金会;

关键词：

ARABIDOPSIS ENDOSPERM; GENOME; GLYCOSYLASE; CHROMATIN; STATES;

D O I：

10.1126/science.1224839

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Arabidopsis thaliana central cell, the companion cell of the egg, undergoes DNA demethylation before fertilization, but the targeting preferences, mechanism, and biological significance of this process remain unclear. Here, we show that active DNA demethylation mediated by the DEMETER DNA glycosylase accounts for all of the demethylation in the central cell and preferentially targets small, AT-rich, and nucleosome-depleted euchromatic transposable elements. The vegetative cell, the companion cell of sperm, also undergoes DEMETER-dependent demethylation of similar sequences, and lack of DEMETER in vegetative cells causes reduced small RNA-directed DNA methylation of transposons in sperm. Our results demonstrate that demethylation in companion cells reinforces transposon methylation in plant gametes and likely contributes to stable silencing of transposable elements across generations.

引用

页码：1360 / 1364

页数：5

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