Development of an antigen ELISA to detect sapovirus in clinical stool specimens

被引：21

作者：

Hansman, GS

Guntapong, R

Pongsuwanna, Y

Natori, K

Katayama, K

Takeda, N

机构：

[1] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan

[2] Minist Publ Hlth, Dept Med Sci, Natl Inst Hlth, Soi Bamrasunaradura, Thailand

来源：

ARCHIVES OF VIROLOGY | 2006年 / 151卷 / 03期

关键词：

D O I：

10.1007/s00705-005-0630-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human sapovirus (SaV) strains are etiological agents of mild and/or acute gastroenteritis in children and adults. In this study, we describe the development of a novel antigen enzyme-linked immunosorbent assay (ELISA) detection system that was based on hyperimmune rabbit and guinea pig antisera raised against SaV genogroup I (GI) virus-like particles. The ELISA had 100% specificity, and sensitivities of 60% and 25% when compared to single-round PCR and nested PCR, respectively. Our results have shown the ELISA was useful in detecting SaV GI antigens in clinical stool specimens collected two days after the onset of illness.

引用

收藏

页码：551 / 561

页数：11

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