Detection of SPM-1-Producing Pseudomonas aeruginosa and Class D β-Lactamase-Producing Acinetobacter baumannii Isolates by Use of Liquid Chromatography-Mass Spectrometry and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：56

作者：

Carvalhaes, Cecilia G. ^{[1
]}

Cayo, Rodrigo ^{[1
]}

Assis, Diego M. ^{[2
]}

Martins, Evelin R. ^{[1
]}

Juliano, Luiz ^{[2
,3
]}

Juliano, Maria A. ^{[2
]}

Gales, Ana C. ^{[1
]}

机构：

[1] Univ Fed Sao Paulo UNIFESP, Dept Med, Lab Alerta, Disciplina Infectol, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo UNIFESP, Dept Biofis, Sao Paulo, Brazil

[3] Fundacao Parque Zool Sao Paulo, Sao Paulo, Brazil

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 01期

关键词：

CARBAPENEMASES;

D O I：

10.1128/JCM.02365-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study evaluates the accuracy of liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for detecting carbapenem hydrolytic activity among SPM-1-, GIM-1-, and GES-5-producing Pseudomonas aeruginosa isolates and OXA-143-, IMP-10-, and OXA-58-producing Acinetobacter baumannii isolates. Class A and B carbapenemase activities were rapidly detected by MALDI-TOF in a 2-h assay. However, an extended incubation time was necessary for detection of carbapenem-hydrolyzing class D beta-lactamase (CHDL) activity in Acinetobacter spp.

引用

页码：287 / 290

页数：4

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