Chemoenzymatic Synthesis of the Immunoglobulin Domain of Tim-3 Carrying a Complex-Type N-Glycan by Using a One-pot Ligation

被引：54

作者：

Asahina, Yuya ^{[1
]}

Kamitori, Shigehiro ^{[2
]}

Takao, Toshifumi ^{[3
]}

Nishi, Nozomu ^{[2
]}

Hojo, Hironobu ^{[1
]}

机构：

[1] Tokai Univ, Inst Glycosci, Dept Appl Biochem, Tokai, Kanagawa 2591292, Japan

[2] Kagawa Univ, Life Sci Res Ctr, Takamatsu, Kagawa 7610793, Japan

[3] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 37期

关键词：

glycoproteins; ligations; peptide thioester; synthesis design; NATIVE CHEMICAL LIGATION; THIOESTER METHOD; GLYCOPEPTIDE LIGATION; POLYPEPTIDE-SYNTHESIS; LINKED OLIGOSACCHARIDES; GLYCOPROTEIN-SYNTHESIS; PEPTIDE-SYNTHESIS; CYSTEINE; PROTEINS; AUXILIARY;

D O I：

10.1002/anie.201303073

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ligations: The title immunoglobin (Ig) domain was chemoenzymatically synthesized. The domain was divided into three segments and N- and S-protected peptide thioesters were synthesized by solid-phase peptide synthesis. The one-pot sequential ligation was achieved by using Ag+-free and Ag+-assisted activation of the thioester. After folding and enzymatic transfer of the synthetic glycan, the desired Ig domain carrying a nonasaccharide was successfully obtained. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9733 / 9737

页数：5

共 57 条

[1]

Aimoto S, 1999, BIOPOLYMERS, V51, P247, DOI 10.1002/(SICI)1097-0282(1999)51:4<247::AID-BIP2>3.0.CO

[2]

2-W

[3]

Bang D., 2006, ANGEW CHEM, V118, P4089

[4] Kinetically controlled ligation for the convergent chemical synthesis of proteins [J].