Isolation of coding sequences from bovine cosmids by means of exon trapping

被引：5

作者：

Comincini, S

Drisaldi, B

Ferretti, L

机构：

[1] CNR, IDVGA, I-20133 MILAN, ITALY

[2] UNIV PAVIA, DEPT GENET & MICROBIOL, I-27100 PAVIA, ITALY

来源：

MAMMALIAN GENOME | 1997年 / 8卷 / 07期

关键词：

D O I：

10.1007/s003359900481

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for the presence of microsatellite markers and physically mapped to chromosomes by FISH. The sequence analysis of 104 clones revealed 18 putative exons, 10 of which showed near identity to known sequences. Among these were the human (cytosine-5)-methyltransferase (DNMT), ATP-citrate lyase (ACLY), the mouse Lbcl1 oncogene, the bovine mitochondrial aconitase (ACO2) and beta-arrestin 1 (ARR1). The chromosomal localization of the cloned exons was inferred from the localization of the parent cosmids. DNMT and ACLY were not previously known in cattle, but the physical localization of the cloned bovine exons is in agreement with the published comparative human and bovine maps. The trapping of exons for bovine ACO2 and ARR1 confirms the available mapping information based on synteny and provides a physical assignment for the genes.

引用

页码：486 / 490

页数：5

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