Stage-specific expression of 14-3-3 in asexual blood-stage Plasmodium

被引:29
作者
Al-Khedery, B [1 ]
Barnwell, JW [1 ]
Galinski, MR [1 ]
机构
[1] NYU, Sch Med, Dept Med & Mol Parasitol, New York, NY 10010 USA
关键词
Plasmodium falciparum; Plasmodium knowlesi; malaria; apicomplexa; 14-3-3; protein; codons;
D O I
10.1016/S0166-6851(99)00090-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper reports the identification of 14-3-3 in Plasmodium. 14-3-3 is an evolutionarily conserved protein that is most noted as a mediator in signal transduction events and cell cycle regulation. The complete cDNA (similar to 2.6 kb) and gDNA (similar to 3.4 kb) of a Plasmodium knowlesi 14-3-3 (Pk14-3-3) is reported. The gene has three introns; two near the beginning and one close to the end of the coding sequence. Also reported, is the gDNA of the Plasmodium falciparum homologue (Pf14-3-3). Unlike in many other organisms, where multiple gene copies and different functional isoforms exist, Plasmodium 14-3-3 is encoded as a single-copy gene. Northern blot analyses show that the Pk14-3-3 transcript in asexual blood stages begins to be expressed in the ring-stage, predominates in young trophozoites, and thereafter declines. An antiserum produced against recombinant Pk14-3-3 reacts via immunoblot and immunoprecipitation with the similar to 30 kDa and the similar to 32 kDa Pk14-3-3 and Pf14-3-3 proteins, respectively. Protein expression in P. knowlesi closely mimics the pattern of the transcript. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:117 / 130
页数:14
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