Identification of a novel selenium metabolite, Se-methyl-N-acetylselenohexosamine, in rat urine by high-performance liquid chromatography-inductively coupled plasma mass spectrometry and -electrospray ionization tandem mass spectrometry

被引:121
作者
Ogra, Y
Ishiwata, K
Takayama, H
Aimi, N
Suzuki, KT [1 ]
机构
[1] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Toxicol & Environm Hlth, Inage Ku, Chiba 2638522, Japan
[2] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Mol Struct & Biol Funct, Inage Ku, Chiba 2638522, Japan
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 767卷 / 02期
关键词
speciation; selenium; 2-acetamide-1,2-dideoxy-beta-D-glucopyranosyl methylselenide;
D O I
10.1016/S1570-0232(01)00581-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The major urinary metabolite of selenium Se) in rats was identified by HPLC-inductively coupled argon plasma mass spectrometry (ICP-MS) and -electrospray tandem mass spectrometry (ESI-MS/MS). As the urine sample was rich in matrices such as sodium chloride and urea, it was partially purified to meet the requirements for ESI-MS. The group of signals corresponding to the So isotope ratio was detected in both the positive and negative ion modes at m/z 300 ([M+H](+)) and 358 ([M+CH3COO](-)) for Se-80, respectively. These results suggested that the molecular mass of the Se metabolite was 299 Da for Se-80. The Se metabolite was deduced to contain one methylselenyl group, one acetyl group and at least two hydroxyl groups from the mass spectra of the fragment ions. The spectrum of the So metabolite was completely identical to that of the synthetic selenosugar, 2-acetamide-1,2-dideoxy-beta-D-glucopyranosyl methylselenide. However, the chromatographic behavior of the Se metabolite was slightly different from that of the synthetic selenosugar. Thus, the major urinary Se metabolite was assigned as a diastereomer of a selenosugar, Se-methyl-N-acetyl-selenohexosamine. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:301 / 312
页数:12
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