Influence of strain and cultivation procedure on the performance of simultaneous saccharification and fermentation of steam pretreated spruce

被引:81
作者
Alkasrawi, M [1 ]
Rudolf, A [1 ]
Lidén, G [1 ]
Zacchi, G [1 ]
机构
[1] Lund Univ, Dept Chem Engn, SE-22100 Lund, Sweden
关键词
strain; cultivation procedure; simultaneous saccharification and fermentation; steam pretreated spruce; ethanol;
D O I
10.1016/j.enzmictec.2005.08.024
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Yeast to be used in simultaneous saccharification and fermentation (SSF) of lignocelluloses materials has to be prepared in a separate cultivation step. The effects of the cultivation procedure on the performance of SSF of steam pretreated softwood were studied in the current work. The yeast used in the SSF was either directly commercially available Baker's yeast (as packaged yeast) or the same strain of yeast produced from the hydrolysate obtained in the pretreatment of the softwood material. A second strain of Saccharomyces cerevisiae TMB3000. isolated from spent sulphite liquor, was also compared with the commercial Baker's yeast. The strains were tested in SSF at substrate loads of 3, 5 and 8% dry weight of water insoluble material. Final ethanol yields were above 85% of the theoretical (based on the available hexoses) in all cases, except for the package yeast for the 8% substrate load, in which case the final yield was less than 65%. The cultivation procedure was found to have a significant impact on the performance during SSF, as well as in small-scale fermentations of hydrolysate liquor without solid material. The Baker's yeast cultivated on the hydrolysate from the steam pretreatment had in all cases a higher productivity, in particular at the highest substrate load. Cultivated Baker's yeast had a slightly higher productivity than TMB3000. The results suggest that the adaptation of the yeast to the inhibitors present in the medium is an important factor that must be considered in the design of SSF processes. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:279 / 286
页数:8
相关论文
共 35 条
[1]   Recirculation of process streams in fuel ethanol production from softwood based on simultaneous saccharification and fermentation [J].
Alkasrawi, M ;
Galbe, M ;
Zacchi, G .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2002, 98 (1-9) :849-861
[2]   IDENTIFICATION OF AROMATIC MONOMERS IN STEAM-EXPLODED POPLAR AND THEIR INFLUENCES ON ETHANOL FERMENTATION BY SACCHAROMYCES-CEREVISIAE [J].
ANDO, S ;
ARAI, I ;
KIYOTO, K ;
HANAI, S .
JOURNAL OF FERMENTATION TECHNOLOGY, 1986, 64 (06) :567-570
[3]  
[Anonymous], 1965, J I BREWING, DOI DOI 10.1002/J.2050-0416.1965.TB02042.X
[4]   THE EFFECTS OF FURFURAL ON ETHANOL-PRODUCTION BY SACCHAROMYCES-CEREVISIAE IN BATCH CULTURE [J].
BOYER, LJ ;
VEGA, JL ;
KLASSON, KT ;
CLAUSEN, EC ;
GADDY, JL .
BIOMASS & BIOENERGY, 1992, 3 (01) :41-48
[5]   ETHANOL FERMENTATION OF CRUDE ACID HYDROLYZATE OF CELLULOSE USING HIGH-LEVEL YEAST INOCULA [J].
CHUNG, IS ;
LEE, YY .
BIOTECHNOLOGY AND BIOENGINEERING, 1985, 27 (03) :308-315
[6]  
CLARK TA, 1984, J CHEM TECH BIOT B, V34, P101
[7]   Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways [J].
Ferreira, BS ;
Calado, CRC ;
van Keulen, F ;
Fonseca, LP ;
Cabral, JMS ;
da Fonseca, MMR .
JOURNAL OF BIOTECHNOLOGY, 2004, 109 (1-2) :159-167
[8]  
HAGGLUND E, 1951, CHEM WOOD, P324
[9]   STEAM-EXPLOSION LIGNINS - FRACTIONATION, COMPOSITION, STRUCTURE AND EXTRACTIVES [J].
HEMMINGSON, JA .
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY, 1987, 7 (04) :527-553
[10]   Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Trametes versicolor [J].
Jönsson, LJ ;
Palmqvist, E ;
Nilvebrant, NO ;
Hahn-Hägerdal, B .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (06) :691-697