The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice

被引:151
作者
Song, JW
Oh, SP
Schrewe, H
Nomura, M
Lei, H
Okano, M
Gridley, T
Li, E
机构
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Med,Cardiovasc Res Ctr, Charlestown, MA 02129 USA
[2] Max Planck Inst Immunbiol, Dept Dev Biol, D-7800 Freiburg, Germany
[3] Jackson Lab, Bar Harbor, ME 04609 USA
关键词
activin receptor; nodal/BMP; gastrulation; mesoderm formation; cyclopia; mouse development;
D O I
10.1006/dbio.1999.9370
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The type II activin receptors, ActRIIA and ActRIIB, have been shown to play critical roles in axial patterning and organ development in mice. To investigate whether their function is required for mesoderm formation and gastrulation as implicated in Xenopus studies, we generated mice carrying both receptor mutations by interbreeding the ActRIIA and ActRIIB knockout mutants. We found that embryos homozygous for both receptor mutations were growth arrested at the egg cylinder stage and did not form mesoderm. further analyses revealed that ActRIIA(-/-)ActRIIB(+/-) and about 15% of the ActRIIA(-/-) embryos failed to form an elongated primitive streak, resulting in severe disruption of mesoderm formation in the embryo proper. Interestingly, we observed similar gastrulation defects in ActRIIA(-/-)nodal(+/-) double mutants, which, if they developed beyond the gastrulation stage, displayed rostral head defects and cyclopia. These results provide genetic evidence that type II activin receptors are required for egg cylinder growth, primitive streak formation, and rostral head development in mice. (C) 1999 Academic Press.
引用
收藏
页码:157 / 169
页数:13
相关论文
共 69 条
  • [51] Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling
    Sampath, K
    Rubinstein, AL
    Cheng, AHS
    Liang, JO
    Fekany, K
    Solnica-Krezel, L
    Korzh, V
    Halpern, ME
    Wright, CVE
    [J]. NATURE, 1998, 395 (6698) : 185 - 189
  • [52] SASAKI H, 1993, DEVELOPMENT, V118, P47
  • [53] EFFECTS OF TRUNCATED ACTIVIN AND FGF RECEPTORS AND OF FOLLISTATIN ON THE INDUCING ACTIVITIES OF BVG1 AND ACTIVIN - DOES ACTIVIN PLAY A ROLE IN MESODERM INDUCTION
    SCHULTEMERKER, S
    SMITH, JC
    DALE, L
    [J]. EMBO JOURNAL, 1994, 13 (15) : 3533 - 3541
  • [54] Shah SB, 1997, DEVELOPMENT, V124, P5127
  • [55] REQUIREMENT FOR LIM1 IN HEAD-ORGANIZER FUNCTION
    SHAWLOT, W
    BEHRINGER, RR
    [J]. NATURE, 1995, 374 (6521) : 425 - 430
  • [56] A VERTEBRATE GENE-RELATED TO ORTHODENTICLE CONTAINS A HOMEODOMAIN OF THE BICOID CLASS AND DEMARCATES ANTERIOR NEUROECTODERM IN THE GASTRULATING MOUSE EMBRYO
    SIMEONE, A
    ACAMPORA, D
    MALLAMACI, A
    STORNAIUOLO, A
    DAPICE, MR
    NIGRO, V
    BONCINELLI, E
    [J]. EMBO JOURNAL, 1993, 12 (07) : 2735 - 2747
  • [57] INDUCING FACTORS IN XENOPUS EARLY EMBRYOS
    SLACK, JMW
    [J]. CURRENT BIOLOGY, 1994, 4 (02) : 116 - 126
  • [58] MESODERM-INDUCING FACTORS IN EARLY VERTEBRATE DEVELOPMENT
    SMITH, JC
    [J]. EMBO JOURNAL, 1993, 12 (12) : 4463 - 4470
  • [59] ACTIVIN AND ITS RECEPTORS DURING GASTRULATION AND THE LATER PHASES OF MESODERM DEVELOPMENT IN THE CHICK-EMBRYO
    STERN, CD
    YU, RT
    KAKIZUKA, A
    KINTNER, CR
    MATHEWS, LS
    VALE, WW
    EVANS, RM
    UMESONO, K
    [J]. DEVELOPMENTAL BIOLOGY, 1995, 172 (01) : 192 - 205
  • [60] PERINATAL LETHALITY AND DEFECTS IN HINDBRAIN DEVELOPMENT IN MICE HOMOZYGOUS FOR A TARGETED MUTATION OF THE ZINC-FINGER GENE KROX20
    SWIATEK, PJ
    GRIDLEY, T
    [J]. GENES & DEVELOPMENT, 1993, 7 (11) : 2071 - 2084