Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

被引：228

作者：

Hocine, Sami ^{[1
]}

Raymond, Pascal ^{[2
]}

Zenklusen, Daniel ^{[2
]}

Chao, Jeffrey A. ^{[1
]}

Singer, Robert H. ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10467 USA

[2] Univ Montreal, Dept Biochem, Montreal, PQ H3C 3J7, Canada

来源：

NATURE METHODS | 2013年 / 10卷 / 02期

基金：

加拿大创新基金会; 加拿大健康研究院; 美国国家卫生研究院;

关键词：

TRANSCRIPTION; TRANSPORT; DYNAMICS; CELL;

D O I：

10.1038/nmeth.2305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

引用

页码：119 / 121

页数：3

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