Cloning and tissue expression of two cDNAs encoding the peroxisomal 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase in the guinea pig liver

被引：7

作者：

Caira, F

CherkaouiMalki, M

Hoefler, G

Latruffe, N

机构：

[1] UNIV BOURGOGNE,LAB BIOL MOL & CELLULAIRE,F-21004 DIJON,FRANCE

[2] GRAZ UNIV,INST PATHOL,A-8036 GRAZ,AUSTRIA

来源：

FEBS LETTERS | 1996年 / 378卷 / 01期

关键词：

bifunctional enzyme; cDNA; 2-enoyl-CoA hydratase 3-hydroxyacyl-CoA dehydrogenase; peroxisome; guinea pig;

D O I：

10.1016/0014-5793(95)01425-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 2-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD) is the second enzyme of the peroxisomal beta-oxidation pathway. In human and rat, only one HD mRNA has been so far detected in the liver. This paper reports for the first time in a mammal species, the guinea pig, the cloning and sequencing of two cDNAs encoding an HD. The 3,274 nucleotide-cDNA is a strictly identical but longer copy of the 2,494 nucleotide-form. A 2,178 bp-open reading frame encodes a protein of 726 amino acids (M(r), 79.3 kDa) with the peroxisomal-targeting signal (tripeptide SKL) at the carboxyterminus. Northern blot analysis of HD mRNA identified three mRNAs of respective sizes 3.5, 2.6 and 1.6 kb in the guinea pig liver and kidneys.

引用

页码：57 / 60

页数：4

共 11 条

[1] THE ANALYSIS OF MODIFIED PEROXISOME PROLIFERATOR RESPONSIVE ELEMENTS OF THE PEROXISOMAL BIFUNCTIONAL ENZYME IN TRANSFECTED HEPG2 CELLS REVEALS 2 REGULATORY MOTIFS [J].