High collection efficiency in fluorescence microscopy with a solid immersion lens

被引：73

作者：

Koyama, K ^{[1
]}

Yoshita, M ^{[1
]}

Baba, M ^{[1
]}

Suemoto, T ^{[1
]}

Akiyama, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Solid State Phys, Chiba 2778581, Japan

来源：

APPLIED PHYSICS LETTERS | 1999年 / 75卷 / 12期

关键词：

D O I：

10.1063/1.124833

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

High efficiency of light collection was demonstrated by applying solid immersion lenses (SILs) with refractive indices n=1.845 and 1.687 to fluorescence microscopy of 0.11-mu m-radius dye-doped polystyrene sphere beads. This was analyzed with theories on the radiation of dipoles near the surface of the dielectric medium. The estimated collection efficiency with the NA=0.8 objective and the n=1.845 SIL was about 60%-70%, which well exceeds the diffraction-limit value of 50% for 2 pi-solid angle collection in conventional methods. (C) 1999 American Institute of Physics. [S0003-6951(99)02438-9].

引用

页码：1667 / 1669

页数：3

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