Synthesis of antisense oligonucleotide-peptide conjugate targeting to GLUT-1 in HepG-2 and MCF-7 cells

被引：27

作者：

Chen, CP

Li, XX

Zhang, LR

Min, JM

Chan, JYW

Fung, KP

Wang, SQ

Zhang, LH ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

[2] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

[3] Beijing Inst Radiat Med, Beijing 100850, Peoples R China

来源：

BIOCONJUGATE CHEMISTRY | 2002年 / 13卷 / 03期

关键词：

D O I：

10.1021/bc015540f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple procedure for the preparation of oligonucleotide-peptide conjugate was developed. p-Hydroxybenzoic acid was used as a linker for the connection of the fragments of peptide and oligonucleotide. It was found that such formed linkage was stable under the conditions of conjugate synthesis. The designed conjugate targeting to GLUT-1 showed up to 50% inhibition of cell proliferation in HepG-2 and MCF-7 cells. Comparing to the results from the expressed antisense RNA in cancer cells, it was proposed that the conjugate of signal peptide mimic and antisense oligonucleotide could improve the permeability of antisense oligonucleotide through cell membrane.

引用

收藏

页码：525 / 529

页数：5

相关论文

共 44 条

[11] ROUTINE PREPARATION OF THIOL OLIGONUCLEOTIDES - APPLICATION TO THE SYNTHESIS OF OLIGONUCLEOTIDE-PEPTIDE HYBRIDS [J].

EDE, NJ ;

TREGEAR, GW ;

HARALAMBIDIS, J .

BIOCONJUGATE CHEMISTRY, 1994, 5 (04) :373-378

[12] SYNTHESIS OF DEFINED PEPTIDE-OLIGONUCLEOTIDE HYBRIDS CONTAINING A NUCLEAR TRANSPORT SIGNAL SEQUENCE [J].

ERITJA, R ;

PONS, A ;

ESCARCELLER, M ;

GIRALT, E ;

ALBERICIO, F .

TETRAHEDRON, 1991, 47 (24) :4113-4120

[13] Synthesis of a nucleopeptide fragment from poliovirus genome [J].

Filippov, D ;

Kuyl-Yeheskiely, E ;

van der Marel, GA ;

Tesser, GI ;

van Boom, JH .

TETRAHEDRON LETTERS, 1998, 39 (21) :3597-3600

[14] FACILE PREPARATION OF NUCLEASE RESISTANT 3' MODIFIED OLIGODEOXYNUCLEOTIDES [J].

GAMPER, HB ;

REED, MW ;

COX, T ;

VIROSCO, JS ;

ADAMS, AD ;

GALL, AA ;

SCHOLLER, JK ;

MEYER, RB .

NUCLEIC ACIDS RESEARCH, 1993, 21 (01) :145-150

[15] THE SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING A 3'-PEPTIDE MOIETY [J].

HARALAMBIDIS, J ;

DUNCAN, L ;

TREGEAR, GW .

TETRAHEDRON LETTERS, 1987, 28 (43) :5199-5202

[16] FACILE PREPARATION OF 3'-OLIGONUCLEOTIDE-PEPTIDE CONJUGATES [J].

JUBY, CD ;

RICHARDSON, CD ;

BROUSSEAU, R .

TETRAHEDRON LETTERS, 1991, 32 (07) :879-882

[17] COVALENT INTERACTION OF PROTEINS AND NUCLEIC-ACIDS - SYNTHETIC AND NATURAL NUCLEOTIDE-PEPTIDES [J].

JUODKA, BA .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 1984, 3 (05) :445-483

[18] A NEW CLASS OF ANTIVIRALS - ANTISENSE OLIGONUCLEOTIDES COMBINED WITH A HYDROPHOBIC SUBSTITUENT EFFECTIVELY INHIBIT INFLUENZA-VIRUS REPRODUCTION AND SYNTHESIS OF VIRUS-SPECIFIC PROTEINS IN MDCK CELLS [J].

KABANOV, AV ;

VINOGRADOV, SV ;

OVCHARENKO, AV ;

KRIVONOS, AV ;

MELIKNUBAROV, NS ;

KISELEV, VI ;

SEVERIN, ES .

FEBS LETTERS, 1990, 259 (02) :327-330

[19] MODIFICATION OF ANTISENSE PHOSPHODIESTER OLIGODEOXYNUCLEOTIDES BY A 5' CHOLESTERYL MOIETY INCREASES CELLULAR-ASSOCIATION AND IMPROVES EFFICACY [J].

KRIEG, AM ;

TONKINSON, J ;

MATSON, S ;

ZHAO, QY ;

SAXON, M ;

ZHANG, LM ;

BHANJA, U ;

YAKUBOV, L ;

STEIN, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (03) :1048-1052

[20] Synthesis of spin labeled conjugate of peptide and peptide nucleic acid [J].

Li, XX ;

Cheng, YZ ;

Zhang, LG ;

Zhang, LH .

SYNTHETIC COMMUNICATIONS, 1999, 29 (09) :1519-1525

← 1 2 3 4 5 →