Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

被引：16

作者：

Nitahara, Satoshi ^{[1
]}

Maeki, Masatoshi ^{[2
]}

Yamaguchi, Hiroshi ^{[1
,3
]}

Yamashita, Kenichi ^{[1
,2
]}

Miyazaki, Masaya ^{[1
,2
]}

Maeda, Hideaki ^{[1
,4
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Measurement Solut Res Ctr, Tosu, Saga 8410052, Japan

[2] Kyushu Univ, Interdisciplinary Grad Sch Engn Sci, Dept Mol & Mat Sci, Kasuga, Fukuoka 8168580, Japan

[3] Tokai Univ, Liberal Arts Educ Ctr, Kumamoto 8691404, Japan

[4] Japan Sci & Technol Agcy JST, CREST, Kawaguchi, Saitama 3320012, Japan

来源：

ANALYST | 2012年 / 137卷 / 24期

关键词：

LASER-INDUCED NUCLEATION; X-RAY-DIFFRACTION; HANGING-DROP; CRYSTALLIZATION CONDITIONS; LYSOZYME CONCENTRATION; MICROFLUIDICS; GROWTH; MICROCHANNEL; SURFACES; KINETICS;

D O I：

10.1039/c2an35942a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Confocal Raman spectroscopic imaging has been used to find the location of protein crystals deposited in a nanodroplet. The depth of the protein crystal has been clearly identified by comparing the three-dimensional Raman spectroscopic images of the protein with those of water. Additionally, the low concentration region around a growing protein crystal in the nanodroplet was visualized using two-dimensional Raman spectroscopic imaging.

引用

页码：5730 / 5735

页数：6

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