Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system

被引：14

作者：

Gerdts, Cory J. ^{[1
,2
]}

Stahl, Glenn L. ^{[1
]}

Napuli, Alberto ^{[3
,4
]}

Staker, Bart ^{[1
,3
]}

Abendroth, Jan ^{[1
,3
]}

Edwards, Thomas E. ^{[1
,3
]}

Myler, Peter ^{[3
]}

Van Voorhis, Wesley ^{[4
]}

Nollert, Peter ^{[2
,5
]}

Stewart, Lance J. ^{[1
,2
,3
]}

机构：

[1] Emerald BioStruct Inc, Bainbridge Isl, WA 98110 USA

[2] Emerald BioSyst Inc, Bainbridge Isl, WA 98110 USA

[3] Seattle Struct Genom Ctr Infect Dis, Seattle, WA USA

[4] Univ Washington, Seattle, WA 98195 USA

[5] Seattle BioMed, Seattle, WA 98109 USA

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2010年 / 43卷

关键词：

protein crystals; microfluids; plugs; genomics; MEMBRANE-PROTEINS; MICROFLUIDICS; MICROBATCH; DIFFUSION;

D O I：

10.1107/S0021889810027378

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The Microcapillary Protein Crystallization System (MPCS) is a microfluidic, plug-based crystallization technology that generates X-ray diffraction-ready protein crystals in nanolitre volumes. In this study, 28 out of 29 (93%) proteins crystallized by traditional vapor diffusion experiments were successfully crystallized by chemical gradient optimization experiments using the MPCS technology. In total, 90 out of 120 (75%) protein/precipitant combinations leading to initial crystal hits from vapor diffusion experiments were successfully crystallized using MPCS technology. Many of the resulting crystals produced high-quality X-ray diffraction data, and six novel protein structures that were derived from crystals harvested from MPCS CrystalCards are reported.

引用

页码：1078 / 1083

页数：6

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