Comparison of supersaturation profiles employed on lysozyme crystallization from a hanging drop

被引:11
作者
Schwartz, AM
Berglund, KA [1 ]
机构
[1] Michigan State Univ, Dept Chem Engn, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA
关键词
D O I
10.1021/cg005514o
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Fiber optic Raman spectroscopy combined with a partial least-squares regression model was used as a monitor of lysozyme concentration during crystallization in a hanging-drop experiment in real time. Raman spectral features of the buffer and protein were employed to build the regression model. The use of fiber optic technology coupled with Raman spectroscopy, which is ideal for use with aqueous solutions, results in a powerful, noninvasive probe of the changing environment within the solution. Monitoring the concentration changes of the lysozyme within the hanging drop permits a measurement of the level of supersaturation of the system and provides for the potential of dynamic control of the crystallization process. Previously, hanging-drop experiments have been monitored in real time. These experiments have given insight into the changing environment of the hanging drop as the lysozyme within the hanging drop concentrates and nucleates and as crystal growth continues. Upon alteration of the ionic strength of the reservoir, the number, size, and quality of the resultant crystals has been affected. This investigation compares the resultant supersaturation of the lysozyme crystallization within the hanging drop by employing various reservoir conditions. These conditions include a constant ionic strength reservoir, a step change in reservoir ionic strength, and a differential change in reservoir ionic strength.
引用
收藏
页码:81 / 85
页数:5
相关论文
共 17 条
[11]   Nucleation and crystallization of globular proteins - What we know and what is missing [J].
Rosenberger, F ;
Vekilov, PG ;
Muschol, M ;
Thomas, BR .
JOURNAL OF CRYSTAL GROWTH, 1996, 168 (1-4) :1-27
[12]   Protein crystallization [J].
Rosenberger, F .
JOURNAL OF CRYSTAL GROWTH, 1996, 166 (1-4) :40-54
[13]   The use of Raman spectroscopy for in situ monitoring of lysozyme concentration during crystallization in a hanging drop [J].
Schwartz, AM ;
Berglund, KA .
JOURNAL OF CRYSTAL GROWTH, 1999, 203 (04) :599-603
[14]   In situ monitoring and control of lysozyme concentration during crystallization in a hanging drop [J].
Schwartz, AM ;
Berglund, KA .
JOURNAL OF CRYSTAL GROWTH, 2000, 210 (04) :753-760
[15]   In situ measurement and dynamic control of the evaporation rate in vapor diffusion crystallization of proteins [J].
Shu, ZY ;
Gong, HY ;
Bi, RC .
JOURNAL OF CRYSTAL GROWTH, 1998, 192 (1-2) :282-289
[16]   Crystal growth and solubility studies of Aspergillus niger acid proteinase A in the presence of both a salt and an organic solvent [J].
Soga, T ;
Sasaki, H ;
Tanokura, M ;
Ataka, M .
JOURNAL OF CRYSTAL GROWTH, 1999, 196 (2-4) :291-296
[17]   Crystallization of recombinant cyclo-oxygenase-2 [J].
Stevens, AM ;
Pawlitz, JL ;
Kurumbail, RG ;
Gierse, JK ;
Moreland, KT ;
Stegeman, RA ;
Loduca, JY ;
Stallings, WC .
JOURNAL OF CRYSTAL GROWTH, 1999, 196 (2-4) :350-355