Small molecule screening by imaging

被引：56

作者：

Eggert, Ulrike S. ^{[1
]}

Mitchison, Timothy J. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2006年 / 10卷 / 03期

关键词：

D O I：

10.1016/j.cbpa.2006.04.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Useful small molecule tools can be discovered in imaging screens that measure phenotype in single cells or small organisms. Recent examples include identification of small molecule inhibitors of processes such as cell migration, cytokinesis, mitotic spindle length determination, rnelanogenesis, aggresome formation, membrane transport and nuclear export. Imaging screens are currently limited by challenges in the areas of image analysis and target identification. We discuss the use of model organisms such as zebrafish in screens and review different methods of target identification. The emerging field of automated image analysis is also introduced.

引用

页码：232 / 237

页数：6

共 35 条

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