A micropatterned hydrogel platform for chemical synthesis and biological analysis

被引：39

作者：

Zourob, M ^{[1
]}

Gough, JE ^{[1
]}

Ulijn, RV ^{[1
]}

机构：

[1] Univ Manchester, Sch Mat, Manchester M1 7HS, Lancs, England

来源：

ADVANCED MATERIALS | 2006年 / 18卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1002/adma.200501840

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A polymer hydrogel platform for peptide arrays compatible both with solid-phase peptide synthesis (SPPS) conditions and the aqueous environment essential for biological assays is demonstrated. Micropatterning followed by multiple-step peptide synthesis in situ is followed by proof-of-concept on-chip biological assays involving cells and enzymes (see Figure and cover).

引用

页码：655 / +

页数：6

共 28 条

[1] Protein microchips: Use for immunoassay and enzymatic reactions [J].

Arenkov, P ;

Kukhtin, A ;

Gemmell, A ;

Voloshchuk, S ;

Chupeeva, V ;

Mirzabekov, A .

ANALYTICAL BIOCHEMISTRY, 2000, 278 (02) :123-131

[2] Biological microchips with hydrogel-immobilized nucleic acids, proteins, and other compounds: Properties and applications in genomics [J].

Barsky, VE ;

Kolchinsky, AM ;

Lysov, YP ;

Mirzabekov, AD .

MOLECULAR BIOLOGY, 2002, 36 (04) :437-455

[3] Protein-acrylamide copolymer hydrogels for array-based detection of tyrosine kinase activity from cell lysates [J].

Brueggemeier, SB ;

Wu, D ;

Kron, SJ ;

Palecek, SP .

BIOMACROMOLECULES, 2005, 6 (05) :2765-2775

[4] Profiling primary protease specificity by peptide synthesis on a solid support [J].

Doezé, RHP ;

Maltman, BA ;

Egan, CL ;

Ulijn, RV ;

Flitsch, SL .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (24) :3138-3141

[5] The SPOT synthesis technique - Synthetic peptide arrays on membrane supports - principles and applications [J].

Frank, R .

JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 267 (01) :13-26

[6] Understanding enzyme action on immobilised substrates [J].

Halling, PJ ;

Ulijn, RV ;

Flitsch, SL .

CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (04) :385-392

[7] RGD modified polymers: biomaterials for stimulated cell adhesion and beyond [J].

Hersel, U ;

Dahmen, C ;

Kessler, H .

BIOMATERIALS, 2003, 24 (24) :4385-4415

[8] Protein surface patterning using nanoscale PEG hydrogels [J].

Hong, Y ;

Krsko, P ;

Libera, M .

LANGMUIR, 2004, 20 (25) :11123-11126

[9] Universal route to cell micropatterning using an amphiphilic comb polymer [J].

Hyun, JH ;

Ma, HW ;

Zhang, ZP ;

Beebe, TP ;

Chilkoti, A .

ADVANCED MATERIALS, 2003, 15 (7-8) :576-579

[10] Semi-wet peptide/protein array using supramolecular hydrogel [J].

Kiyonaka, S ;

Sada, K ;

Yoshimura, I ;

Shinkai, S ;

Kato, N ;

Hamachi, I .

NATURE MATERIALS, 2004, 3 (01) :58-64

← 1 2 3 →