Endogenous cell repair of chronic demyelination

被引:104
作者
Armstrong, Regina C.
Le, Tuan Q.
Flint, Nicole C.
Vana, Adam C.
Zhou, Yong-Xing
机构
[1] Uniformed Serv Univ Hlth Sci, Dept Anat Physiol & Genet, Bethesda, MD 20814 USA
[2] Uniformed Serv Univ Hlth Sci, Program Neurosci, Bethesda, MD 20814 USA
关键词
cuprizone; demyelinating disease; differentiation; fibroblast growth factor; myelin repair; oligodendrocyte progenitor;
D O I
10.1097/01.jnen.0000205142.08716.7e
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
in multiple sclerosis lesions, remyelination typically fails with repeated or chronic demyelinating episodes and results in neurologic disability. Acute demyclination models in rodents typically exhibit robust spontaneous remyelination that prevents appropriate evaluation of strategies for improving conditions of insufficient remyelination. In the current study, we used a mouse model of chronic demyelination induced by continuous ingestion of 0.2% cuprizone for 12 weeks. This chronic process depleted the oligodendrocyte progenitor population and impaired oligodendrocyte regeneration. Remyelination remained limited after removal of cuprizone from the diet. Fibroblast growth factor 2 (FGF2) expression was persistently increased in the corpus callosum of chronically demyelinated mice as compared with nonlesioned mice. We used FGF2(-/-) mice to determine whether removal of endogenous FGF2 promoted remyelination of chronically demyelinated areas. Wild-type and FGF2(-/-) mice exhibited similar demyelination during chronic cuprizone treatment. Importantly, in contrast to wild-type mice, the FGF2(-/-) mice spontaneously remyelinated completely during the recovery period after chronic demyelination. Increased remyelination in FGF2(-/-) mice correlated with enhanced oligodendroglial regeneration. FGF2 genotype did not alter the density of oligodendrocyte progenitor cells or proliferating cells after chronic demyelination. These findings indicate that attenuating FGF2 created a sufficiently permissive lesion environment for endogenous cells to effectively remyelinate viable axons even after chronic demyclination.
引用
收藏
页码:245 / 256
页数:12
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