Different myrosinase and idioblast distribution in Arabidopsis and Brassica napus

被引:159
作者
Andréasson, E
Jorgensen, LB
Höglund, AS
Rask, L
Meijer, J
机构
[1] Univ Copenhagen, Inst Mol Biol, Dept Plant Physiol, DK-1353 Copenhagen, Denmark
[2] Univ Copenhagen, Inst Bot, Dept Evolut Bot, DK-1123 Copenhagen, Denmark
[3] Swedish Univ Agr Sci, Uppsala Genet Ctr, Dept Plant Biol, S-75007 Uppsala, Sweden
[4] Univ Uppsala, Ctr Biomed, Dept Med Biochem & Microbiol, S-75123 Uppsala, Sweden
关键词
D O I
10.1104/pp.010334
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Myrosinase (EC 3.2.3.1) is a glucosinolate-degrading enzyme mainly found in special idioblasts, myrosin cells, in Brassicaceae. This two-component system of secondary products and degradative enzymes is important in plant-insect interactions. Immunocytochemical analysis of Arabidopsis localized myrosinase exclusively to myrosin cells in the phloem parenchyma, whereas no myrosin cells were detected in the ground tissue. In Brassica napus, myrosinase could be detected in myrosin cells both in the phloem parenchyma and in the ground tissue. The myrosin cells were similar in Arabidopsis and B. napus and were found to be different from the companion cells and the glucosinolate-containing S-cells present in Arabidopsis. Confocal laser scanning immunomicroscopy analysis of myrosin cells in B. napus embryos showed that the myrosin grains constitute a continuous reticular system in the cell. These findings indicate that in the two species studied, initial cells creating the ground tissue have different potential for making idioblasts and suggest that the myrosinase-glucosinolate system has at least partly different functions. Several myrosinases in B. napus extracts are recovered in complex together with myrosinase-binding protein (MBP), and the localization of MBP was therefore studied in situ. The expression of MBP was highest in germinating seedlings of B. napus and was found in every cell except the myrosin cells of the ground tissue. Rapid disappearance of the MBP from the non-myrosin cells and emergence of MBP in the myrosin cells resulted in an apparent colocalization of MBP and myrosinase in 7-d-old seedlings.
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页码:1750 / 1763
页数:14
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