Photobleaching under single photon and multi-photon excitation:: chloroplasts in protoplasts from Arabidopsis thaliana

被引：8

作者：

Kao, FJ ^{[1
]}

Wang, YM

Chen, JC

Cheng, PC

Chen, RW

Lin, BL

机构：

[1] Natl Sun Yat Sen Univ, Ctr Neurosci, Kaohsiung 80424, Taiwan

[2] Natl Sun Yat Sen Univ, Dept Phys, Kaohsiung 80424, Taiwan

[3] SUNY Buffalo, Dept Elect Engn, Buffalo, NY 14260 USA

[4] Acad Sinica, Inst Mol Biol, Taipei 11529, Taiwan

来源：

OPTICS COMMUNICATIONS | 2002年 / 201卷 / 1-3期

关键词：

micro-spectroscopy; multi-photon excitation; photobleaching; chloroplasts;

D O I：

10.1016/S0030-4018(01)01679-0

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 91

页数：7

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