Isolation of intact collagen fibrils from healing ligament

被引:24
作者
DeVente, JE
Lester, GE
Trotter, JA
Dahners, LE
机构
[1] UNIV N CAROLINA,SCH MED,DEPT ORTHOPAED SURG,CHAPEL HILL,NC 27599
[2] UNIV NEW MEXICO,SCH MED,DEPT ORTHOPAED & REHABIL,ALBUQUERQUE,NM 87131
来源
JOURNAL OF ELECTRON MICROSCOPY | 1997年 / 46卷 / 04期
关键词
collagen; ligament; fibrils; healing; gentamicin; isolation; INVITRO; SEGMENTS; TIPS;
D O I
10.1093/oxfordjournals.jmicro.a023530
中图分类号
TH742 [显微镜];
学科分类号
摘要
The inability to isolate intact collagen fibrils has limited the study of their growth and structure. Although intact fibrils have been isolated from echinoderms and from embryonic chick tissues, no method has previously succeeded in isolating intact collagen fibrils from a postfoetal vertebrate tissue. Having previously observed that gentamicin weakens rat tail tendon, we hypothesized that gentamicin may weaken interfibrillar bonds and that intact collagen fibrils might be isolated from tissue treated with gentamicin. In this study medial collateral knee ligaments of Sprague Dawley rats were transected and then harvested 24, 48 or 96 h postoperatively. These specimens were placed in gentamicin or phosphate-buffered saline for 72 h, vortexed for 1 h, incubated in gentamicin for an additional 24 h, and vortexed again for 1 h. Negatively stained specimens were examined with a transmission electron microscope. The phosphate-buffered saline specimens yielded only broken fibrils. The,gentamicin specimens yielded both broken and intact fibrils. The latter had tapering ends and consisted of molecules orientated such that their amino termini pointed toward the tip and their carboxy termini pointed toward a short central region where the molecular polarity reversed.
引用
收藏
页码:353 / 356
页数:4
相关论文
共 16 条
[11]  
PIEZ KA, 1959, BIOCHIM BIOPHYS ACTA, V34, P24
[12]  
Piez KA., 1984, EXTRACELLULAR MATRIX, P1
[13]   NATIVE COLLAGEN FIBRILS FROM ECHINODERMS ARE MOLECULARLY BIPOLAR [J].
THURMOND, FA ;
TROTTER, JA .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 235 (01) :73-79
[14]   MOLECULAR-STRUCTURE AND FUNCTIONAL-MORPHOLOGY OF ECHINODERM COLLAGEN FIBRILS [J].
TROTTER, JA ;
THURMOND, FA ;
KOOB, TJ .
CELL AND TISSUE RESEARCH, 1994, 275 (03) :451-458
[15]  
TROTTER JA, 1989, CELL TISSUE RES, V258, P527
[16]  
WILLIAMS BR, 1978, J BIOL CHEM, V253, P6578