Linkage mapping of rat Chromosome 5 markers generated from chromosome-specific libraries

被引：9

作者：

Lan, H ^{[1
]}

Shepel, LA ^{[1
]}

Haag, JD ^{[1
]}

Gould, MN ^{[1
]}

机构：

[1] Univ Wisconsin, Sch Med, McArdle Lab Canc Res, Madison, WI 53706 USA

来源：

MAMMALIAN GENOME | 1999年 / 10卷 / 07期

关键词：

D O I：

10.1007/s003359901071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seventy-six novel microsatellite markers with various simple sequence repeat (SSR) motifs are reported in this paper. They were generated on the basis of non-radioactive library screening procedures from flow-sorted rat Chromosome (Chr) 5-specific DNA, and were mapped in three rat backcross populations. Fifty-four of these markers mapped to Chr 5, while the other 22 mapped to other chromosomes of the rat genome. The marker D3Uwm8 is a new microsatellite marker for the rat syndecan 4 (ryudocan) gene. A genotyping protocol based on agarose gel electrophoresis is also provided in this paper.

引用

页码：687 / 691

页数：5

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