Nano-Chemical Infrared Imaging of Membrane Proteins in Lipid Bilayers

被引：101

作者：

Berweger, Samuel ^{[1
,2
,5
,6
]}

Nguyen, Duc M. ^{[3
]}

Muller, Eric A. ^{[1
,2
,5
,6
]}

Bechtel, Hans A. ^{[7
]}

Perkins, Thomas T. ^{[4
,5
,6
]}

Raschke, Markus B. ^{[1
,2
,5
,6
]}

机构：

[1] Univ Colorado, Dept Phys, Boulder, CO 80309 USA

[2] Univ Colorado, Dept Chem, Boulder, CO 80309 USA

[3] Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA

[4] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

[5] Univ Colorado, NIST, Joint Inst Lab Astrophys, Boulder, CO 80309 USA

[6] Univ Colorado, Boulder, CO 80309 USA

[7] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Adv Light Source Div, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 49期

基金：

美国国家科学基金会;

关键词：

ATOMIC-FORCE MICROSCOPY; NEAR-FIELD MICROSCOPY; OPTICAL MICROSCOPY; PURPLE MEMBRANES; SPECTROSCOPY; RESOLUTION; BACTERIORHODOPSIN; LIMIT; SCATTERING; STABILITY;

D O I：

10.1021/ja409815g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone, we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nanospectroscopy, with potential extension to a wide range of biomolecular systems.

引用

页码：18292 / 18295

页数：4

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