Macrolide Antibiotic-Mediated Downregulation of MexAB-OprM Efflux Pump Expression in Pseudomonas aeruginosa

被引：18

作者：

Sugimura, Makoto ^{[2
]}

Maseda, Hideaki ^{[3
]}

Hanaki, Hideaki ^{[1
]}

Nakae, Taiji ^{[1
]}

机构：

[1] Kitasato Inst Univ, Lab Antiinfect Drugs, Sagamihara, Kanagawa 2288555, Japan

[2] Kitasato Inst Univ, Grad Sch Med Sci, Sagamihara, Kanagawa 2288555, Japan

[3] Fac Engn, Dept Biol Sci & Technol, Tokushima 7708605, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2008年 / 52卷 / 11期

关键词：

D O I：

10.1128/AAC.00511-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Macrolide antibiotics modulate the quorum-sensing system of Pseudomonas aeruginosa. We tested the effect of macrolide antibiotics on the cell density-dependent expression of the MexAB-OprM efflux pump and found that 1.0 mu g/ml (MIC/6.25) of azithromycin suppressed the expression of MexAB-OprM by about 70%, with the result that the cells became two- to fourfold more susceptible to antibiotics such as aztreonam, tetracycline, carbenicillin, chloramphenicol, and novobiocin.

引用

页码：4141 / 4144

页数：4

共 32 条

[21] Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1 [J].

Nouwens, AS ;

Beatson, SA ;

Whitchurch, CB ;

Walsh, BJ ;

Schweizer, HP ;

Mattick, JS ;

Cordwell, SJ .

MICROBIOLOGY-SGM, 2003, 149 :1311-1322

[22] Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals [J].

Pearson, JP ;

Van Delden, C ;

Iglewski, BH .

JOURNAL OF BACTERIOLOGY, 1999, 181 (04) :1203-1210

[23] Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa [J].

Pesci, EC ;

Milbank, JBJ ;

Pearson, JP ;

McKnight, S ;

Kende, AS ;

Greenberg, EP ;

Iglewski, BH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11229-11234

[24]

Poole K, 2001, J MOL MICROB BIOTECH, V3, P255

[25] nalB-type mutations causing the overexpression of the MexAB-OprM efflux pump are located in the mexR gene of the Pseudomonas aeruginosa chromosome [J].

Saito, K ;

Yoneyama, H ;

Nakae, T .

FEMS MICROBIOLOGY LETTERS, 1999, 179 (01) :67-72

[26] A quorum-sensing autoinducer enhances the mexAB-oprM efflux-pump expression without the MexR-mediated regulation in Pseudomonas aeruginosa [J].

Sawada, I ;

Maseda, H ;

Nakae, T ;

Uchiyama, H ;

Nomura, N .

MICROBIOLOGY AND IMMUNOLOGY, 2004, 48 (05) :435-439

[27] Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes:: a transcriptome analysis [J].

Schuster, M ;

Lostroh, CP ;

Ogi, T ;

Greenberg, EP .

JOURNAL OF BACTERIOLOGY, 2003, 185 (07) :2066-2079

[28] 2 PLASMIDS, X1918 AND Z1918, FOR EASY RECOVERY OF THE XYLE AND LACZ REPORTER GENES [J].

SCHWEIZER, HP .

GENE, 1993, 134 (01) :89-91

[29] 'Subinhibitory' erythromycin represses production of Pseudomonas aeruginosa lectins, autoinducer and virulence factors [J].

Sofer, D ;

Gilboa-Garber, N ;

Belz, A ;

Garber, NC .

CHEMOTHERAPY, 1999, 45 (05) :335-341

[30] Regulatory effects of macrolides on bacterial virulence: Potential role as quorum-sensing inhibitors [J].

Tateda, K ;

Standiford, TJ ;

Pechere, JC ;

Yamaguchi, K .

CURRENT PHARMACEUTICAL DESIGN, 2004, 10 (25) :3055-3065

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