Netrin UNC-6 and the regulation of branching and extension of motoneuron axone from the ventral nerve gord of Caenorhabditis elegans

被引:36
作者
Lim, YS [1 ]
Mallapur, S [1 ]
Kao, GT [1 ]
Ren, XC [1 ]
Wadsworth, WG [1 ]
机构
[1] Robert Wood Johnson Med Sch, Dept Pathol, Piscataway, NJ 08854 USA
关键词
netrin; UNC-6; C; elegans; guidance; axon branching; genetics; invertebrate; in vivo;
D O I
10.1523/JNEUROSCI.19-16-07048.1999
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the Caenorhabditis elegans embryo, some ventral midline motoneurons extend a process circumferentially to the dorsal midline and a process longitudinally along ventral nerve cord interneurons. Circumferential migrations are guided by netrin UNC-6, which repels motoneuron axons dorsally. Although the motoneuron cell bodies and the longitudinal axons are positioned along UNC-6-expressing interneurons in the ventral nerve cord, the circumferential processes extend only from the motoneuron cell bodies and from defined locations along some longitudinal axons. This implies a mechanism regulates motoneuron branching of UNC-6-responsive processes. We show that expression of unc-6 Delta C, which encodes UNC-6 without domain C, partially rescues circumferential migration defects in unc-6 null animals. This activity depends on the netrin receptors UNC-5 and UNC-40. These results indicate that UNC-6 Delta C can provide the circumferential guidance functions of UNC-6. Furthermore, we show that expression of unc-6 Delta C causes motoneuron branching and the extension of processes from abnormal positions along the ventral nerve cord. This activity is also UNC-5- and UNC-40-dependent, We propose that local interactions mediated by domain C regulate motoneuron branching and responsiveness to the UNC-6 cue.
引用
收藏
页码:7048 / 7056
页数:9
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