Comparison of esterification and transesterification of fructose by porcine pancreas lipase immobilized on different supports

被引:13
作者
Bagi, K [1 ]
Simon, LM [1 ]
机构
[1] Attila Jozsef Univ, Dept Biochem, H-6726 Szeged, Hungary
关键词
esterification; fructose; immobilized; porcine pancreas lipase;
D O I
10.1023/A:1008980809993
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The syntheses of fructose butyrate with five different immobilized porcine pancreas lipases were studied in acetonitrile with butyric acid and tributyrin as acyl donors. The hydrolytic activities of the enzymes were measured during the syntheses. The transesterification yield was the highest with the Celite-adsorbed enzyme: 57% (1.42 g fructose butyrate l(-1)), and this form exhibited the highest degree of hydrolytic activity preservation (57%) after operation for 20 days.
引用
收藏
页码:309 / 312
页数:4
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