Estimation of temperature dependent growth rate and lag time of Listeria monocytogenes by optical density measurements

被引:69
作者
Augustin, JC
Rosso, L
Carlier, V
机构
[1] Ecole Natl Vet Alfort, Serv Hyg & Ind Denrees Alimentaires Origine Anim, F-94704 Maisons Alfort, France
[2] Pole Competence Secur Aliments, Grp Danone, F-19100 Brive La Gaillarde, France
关键词
automated turbidimetry; lag time; Listeria monocytogenes; maximum specific growth rate; predictive microbiology;
D O I
10.1016/S0167-7012(99)00089-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An automated turbidimetric system, Bioscreen C, was used to monitor growth of ten strains of Listeria monocytogenes at different temperatures. Several methods for estimation of maximum specific growth rate (mu(max)) and lag time (lag) from turbidimetric data were compared to values estimated from viable count data. By using a calibration factor, reliable estimations of mu(max) could be obtained from turbidimetric measurements. On the other hand, accurate estimations of lag required some viable count data. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:137 / 146
页数:10
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