Nano-calorimetry of small-sized biological samples

被引：46

作者：

Lerchner, J. ^{[1
]}

Wolf, A. ^{[1
]}

Schneider, H. -J. ^{[1
]}

Mertens, F. ^{[1
]}

Kessler, E. ^{[2
]}

Baier, V. ^{[2
]}

Funfak, A. ^{[3
]}

Nietzsch, M. ^{[4
]}

Kruegel, M. ^{[4
]}

机构：

[1] Tech Univ Bergakad Freiberg, Inst Phys Chem, D-09596 Freiberg, Germany

[2] Inst Photon Technol IPHT, D-07742 Jena, Germany

[3] Tech Univ Ilmenau, Inst Phys, D-98684 Ilmenau, Germany

[4] Eurotron Gesell F Wiss Geratebau mbH, D-04229 Leipzig, Germany

来源：

THERMOCHIMICA ACTA | 2008年 / 477卷 / 1-2期

关键词：

Chip-calorimetry; Biofilm; pseudomonas putida; Staphyloccocus aureus; Zebra fish embryos;

D O I：

10.1016/j.tca.2008.08.007

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

A miniaturized calorimeter with a signal noise of less than 10 nW is presented for the first time. The outstanding signal performance was obtained due to an elaborated temperature control inside the system. With a sample volume of around 6 mu l a specific heat flow detection limit of <2 mW l(-1) was achieved, which is a prerequisite for promising microbiological applications. The performance of the new calorimeter is demonstrated by investigations of the metabolic heat production of biofilms, bacteria cultured in suspensions, and single fish embryos. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：48 / 53

页数：6

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